MACSPlex Cytokine Kits

MACSPlex Cytokine Kits

MACSPlex Assays are designed for determining concentrations of soluble analytes in a single sample. The analysis is based on MACSPlex Capture Beads, which display defined fluorescence properties and can be identified using standard flow cytometry techniques.
MACSPlex Capture Beads within the MACSPlex Cytokine 12 Kit, human and MACSPlex Cytokine 10 Kit, mouse contain a cocktail of various fluorescently labeled bead populations, each coated with a specific antibody reacting with one of the respective cytokines within the sample.
In combination with the Express Modes of the MACSQuant
®
Analyzers, the MACSPlex Cytokine Kits are optimized for automated measurement of beads. They simplify flow cytometric analysis via predefined experiment settings as well as acquisition and analysis templates. They apply an automated gating strategy to populations of interest that will be automatically adjusted for each data file individually to achieve optimal results.
MACSPlex Cytokine 12 Capture Beads, human and MACSPlex Cytokine 10 Capture Beads, mouse consist of bead populations that have been coated with capture antibodies specific for cytokines. The bead populations can be distinguished by different fluorescence intensities detected in the FITC (B1) and PE (B2) channel of the MACSQuant
®
Analyzer and MACSQuant Analyzer 10 (as well as respective channels of comparable flow cytometers). MACSPlex Cytokine Capture Beads are added to unknown samples and to serial dilutions of the MACSPlex Cytokine Standard. During a 2-hour incubation period, the cytokines are captured by the MACSPlex Capture Beads. Subsequently, MACSPlex Cytokine Detection Reagent containing a mixture of APC-conjugated anti-cytokine antibodies, is added in order to form sandwich complexes during a 1-hour incubation period. Standard curves for each of the cytokines are generated. The median of the APC fluorescence of each capture bead population gives the concentration of each cytokine in the unknown samples.

Applications

MACSPlex Cytokine 12 Kit, human has been developed for the simultaneous flow cytometric detection of the soluble human cytokines GM-CSF, IFN-α, IFN-γ, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-17A, and TNF-α in a single sample. The kit has been optimized for use with serum, plasma, and cell culture supernatants.
MACSPlex Cytokine 10 Kit, mouse has been developed for the simultaneous flow cytometric detection of the soluble mouse cytokines GM-CSF, IFN-γ, IL-2, IL-4, IL-5, IL-10, IL-12p70, IL-17A, IL-23, and TNF-α in a single sample. The kit has been optimized for use with serum, plasma, and cell culture supernatants.
Product options: 2

Product information

Size
Order no.
Price

MACSPlex Cytokine 10 Kit, mouse

  • For research use only
for 100
tests
130-101-740
-

MACSPlex Cytokine 12 Kit, human

  • For research use only
for 100
tests
130-099-169
-