Mouse IL-4 Secretion Assay – Detection Kit (PE)

Mouse IL-4 Secretion Assay – Detection Kit (PE)

The IL-4 Secretion Assay was developed for the sensitive detection of mouse IL-4-secreting cells.

Background information

Interleukin-4 (IL-4) is predominantly secreted by activated CD4
+
memory and effector T
H
2 cells, basophils, and mast cells. It induces and supports humoral immune responses for the neutralization of extracellular pathogens. The T
H
2 types of immune mechanisms can also be involved in immunologic disorders, such as IgE-mediated allergy.

Downstream applications

The assay has been used for research on T
H
2 development and commitment
1,3
and for evaluation of IL-4 regulation and expression
2,4
.
The Mouse IL-4 Secretion Assay (PE) is suitable for two-color cytokine analysis in combination with the Mouse IFN-γ, IL-2, IL-5, or IL-10 Secretion Assay - Detection Kit (APC).
  • Selected References

    1. Ouyang, W. et al. (2000) Stat6-independent GATA-3 autoactivation directs IL-4-independent Th2 development and commitment. Immunity 12: 27-37
    2. Hu-Li, J. et al. (2001) Regulation of expression of IL-4 alleles: analysis using a chimeric GFP/IL-4 gene. Immunity 14: 1-11
    3. Löhning, M. et al. (2003) Establishment of memory for IL-10 expression in developing T helper 2 cells requires repetitive IL-4 costimulation and does not impair proliferation. Proc. Natl. Acad. Sci. U.S.A. 100: 12307-12312
    4. Guo, L. et al. (2004) Probabilistic regulation of IL-4 production in Tʜ2 cells: accessibility at the Il4 locus. Immunity 20: 193-203
    5. Yang, J. Q. et al. (2003) Immunoregulatory role of CD1d in the hydrocarbon oil-induced model of lupus nephritis. J. Immunol. 171: 2142-2153
Product options: 1

Product information

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Order no.
Price

Mouse IL-4 Secretion Assay – Detection Kit (PE)

  • For research use only
for 100
tests
(1)
130-090-479
-