Mouse IFN-γ Secretion Assay – Detection Kits

Mouse IFN-γ Secretion Assay – Detection Kits

The IFN-γ Secretion Assay was developed for the sensitive detection of mouse IFN-γ-secreting cells.

Background information

Interferon-gamma (IFN-γ) is predominantly secreted by activated CD8
+
and CD4
+
memory and effector T cells as well as by NK cells. It is mainly involved in the regulation of inflammatory immune responses. These T
H
1 types of immune mechanisms are effective against intracellular pathogens and tumors and can be involved in immunological disorders, such as autoimmune reactions.

Downstream applications

The Mouse IFN-γ Secretion Assay was used to analyze the sequential production of cytokines by individual staphylococcal enterotoxin B-activated T helper lymphocytes
1
and for studies on T
H
1 cell differentiation
2,3
. Furthermore, it was used for isolation of tumor-reactive T cells from immunized mice for adoptive T cell transfer experiments
4
and from mice immunized with viral antigens.
5
Mouse NKT cells were analyzed
6,7
and isolated
7
using the IFN-γ Secretion Assay after stimulation with glycolipid antigens. IFN-γ expression by T cells was also analyzed for intrahepatic mononuclear cells
8
and cells from bronchioalveolar lavages
9
.
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