Mouse IFN-γ Secretion Assay – Detection Kits

Mouse IFN-γ Secretion Assay – Detection Kits

The IFN-γ Secretion Assay was developed for the sensitive detection of mouse IFN-γ-secreting cells.

Background information

Interferon-gamma (IFN-γ) is predominantly secreted by activated CD8
+
and CD4
+
memory and effector T cells as well as by NK cells. It is mainly involved in the regulation of inflammatory immune responses. These T
H
1 types of immune mechanisms are effective against intracellular pathogens and tumors and can be involved in immunological disorders, such as autoimmune reactions.

Downstream applications

The Mouse IFN-γ Secretion Assay was used to analyze the sequential production of cytokines by individual staphylococcal enterotoxin B-activated T helper lymphocytes
1
and for studies on T
H
1 cell differentiation
2,3
. Furthermore, it was used for isolation of tumor-reactive T cells from immunized mice for adoptive T cell transfer experiments
4
and from mice immunized with viral antigens.
5
Mouse NKT cells were analyzed
6,7
and isolated
7
using the IFN-γ Secretion Assay after stimulation with glycolipid antigens. IFN-γ expression by T cells was also analyzed for intrahepatic mononuclear cells
8
and cells from bronchioalveolar lavages
9
.
  • Selected references

    1. Dana, R. et al. (2016) IL-17 Augments B Cell Activation in Ocular Surface Autoimmunity. J. Immunol. 197(9): 3464-3470
    2. Krilis, S. A. et al. (2016) CD117+ Dendritic and Mast Cells Are Dependent on RasGRP4 to Function as Accessory Cells for Optimal Natural Killer Cell-Mediated Responses to Lipopolysaccharide. PLoS One 11(3)
    3. Assenmacher et al. (1998) Sequential production of IL-2, IFN-gamma and IL-10 by individual staphylococcal enterotoxin B-activated T helper lymphocytes. Eur. J. Immunol. 28: 1534-1543
    4. Wu, C. Y. et al. (2002) Distinct lineages of T(H)1 cells have differential capacities for memory cell generation in vivo. Nat. Immunol. 3: 852-858
    5. Zhang, Y. et al. (2001) Interferon gamma stabilizes the T helper cell type 1 phenotype. J. Exp. Med. 194: 165-172
    6. Becker, C. et al. (2001) Adoptive tumor therapy with T lymphocytes enriched through an IFN-gamma capture assay. Nat. Med. 7: 1159-1162
    7. Riberdy, J. M. et al. (2001) Cutting edge: culture with high doses of viral peptide induces previously unprimed CD8(+) T cells to produce cytokine. J. Immunol. 167: 2437-2440
    8. Hayakawa et al. (2001) Critical contribution of IFN-gamma and NK cells, but not perforin-mediated cytotoxicity, to anti-metastatic effect of alpha-galactosylceramide. Eur. J. Immunol. 31: 1720-1727
    9. Yang, J. Q. et al. (2003) Immunoregulatory role of CD1d in the hydrocarbon oil-induced model of lupus nephritis. J. Immunol. 171: 2142-2153
    10. Berenzon, D. et al. (2003)
      Protracted protection to
      Plasmodium berghei
      malaria is linked to functionally and phenotypically heterogeneous liver memory CD8
      +
      T cells.
      J. Immunol. 171: 2024-2034
    11. Mc Allister, F. et al. (2004)
      T cytotoxic-1 CD8
      +
      T cells are effector cells against pneumocystis in mice.
      J. Immunol. 172: 1132-1138
  • Special protocols

Product options: 2
130-090-516

Mouse IFN-γ Secretion Assay – Detection Kit (PE)

for 100
tests
(1)
-
130-090-984

Mouse IFN-γ Secretion Assay – Detection Kit (APC)

for 100
tests
(1)
-