CD154/TNF-α/CD4 Detection Kit, human

CD154/TNF-α/CD4 Detection Kit, human

The kit is suitable for flow cytometric identification and enumeration of TNF-α-producing CD154
+
CD4
+
T cells upon
in vitro
stimulation. Other applications include identification and enumeration of TNF-α-producing, antigen-specific CD154
+
CD4
+
T cells upon restimulation or monitoring of specificity of antigen-specific T cell lines.

Background information

The CD154/TNF-α/CD4 Detection Kit is designed for the intracellular detection of TNF-α production in activated CD4
+
T cells. It contains an antibody recognizing CD154 – a specific marker for T cell activation – and all reagents for the immunofluorescent detection of TNF-α-producing activated CD4
+
T cells from whole blood or PBMCs.
By combining the detection of cytokine production with staining of CD154 and CD4, the sensitive and specific detection of activated antigen-specific CD4
+
T cells is easily possible.
TNF-α is secreted by activated CD4
+
T cells, monocytes, macrophages, NK cells, and neutrophils. TNF-α is a mediator of inflammatory immune responses and is selectively toxic for many transformed cells, especially in combination with IFN-γ.
Product options: 1

Product information

Size
Order no.
Price

CD154/TNF-α/CD4 Detection Kit, human

  • For research use only
for 100
tests
(1)
130-092-813
-