CD154/IL-2/CD4 Detection Kit, human

CD154/IL-2/CD4 Detection Kit, human

The kit is suitable for flow cytometric identification and enumeration of IL-2-producing CD154
+
CD4
+
T cells upon
in vitro
stimulation. Other applications include identification and enumeration of IL-2-producing, antigen-specific CD154
+
CD4
+
T cells upon restimulation or monitoring of specificity of antigen-specific T cell lines.

Background information

The CD154/IL-2/CD4 Detection Kit is designed for the intracellular detection of IL-2 production in activated CD4
+
T cells. It contains an antibody recognizing CD154 – a specific marker for T cell activation – and all reagents necessary for the immunofluorescent detection of IL-2-producing activated CD4
+
T cells from whole blood or PBMCs.
By combining the detection of cytokine production with staining of CD154 and CD4, the sensitive and specific detection of activated antigen-specific CD4
+
T cells is easily possible.
IL-2 is rapidly secreted by naive T helper cells and by certain subsets of memory T cells upon activation. It promotes growth and differentiation of T cells and has pleiotropic effects on many other leukocytes. The quantitative analysis of IL-2-producing cells can provide important information on the course of an immune response.
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