Annexin V-FITC Kit

Annexin V-FITC Kit

This kit is suitable for the identification and enumeration of dead cells, such as apoptotic or necrotic cells, by flow cytometry.

Background information

The Annexin V-FITC Kit contains reagents for the simultaneous fluorescent detection of apoptotic, necrotic, and dead cells through the specific binding of FITC-labeled Annexin V to phosphotidylserine (PS). The kit consists of Annexin V-FITC, Annexin V Binding Buffer, and Propidium Iodide Solution.
In most normal, viable eukaryotic cells, the negatively charged phospholipid PS is located in the cytosolic leaflet of the plasma membrane lipid bilayer.
1
PS redistribution from the inner to the outer leaflet is an early and widespread event during apoptosis.
1,2
However, in necrosis, PS becomes accessible due to the disruption of membrane integrity.
2
Apart from necrosis and apoptosis, PS also becomes accessible in activated platelets
3
, in certain cell anomalies such as sickle cell anemia
4
, in erythrocyte senescence
5
, upon degranulation of mast cells
6
, and in certain stages of B cell differentiation
7
. PS exposure by apoptotic cells serves as a trigger for the recognition and removal by macrophages.
8,9
Annexin V is a 35 kDa phospholipid-binding protein and has a high affinity for PS in the presence of physiological concentrations of calcium (Ca
2+
).
10
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Annexin V-FITC Kit

  • For research use only
for
up to
100
test
(1)
130-092-052
-

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