REAlease® CD8a Complexes, mouse

REAlease
®
CD8a Complexes
, mouse

Clone: REAL137 | Dilution: 1:50
Clone REAL137 is an antibody fragment derived from the full CD8a antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL137 is specific for the mouse CD8a antigen, also known as LY-2 or T8, which is expressed on cytotoxic T cells and at lower levels on subpopulations of dendritic cells and TCRγ/δ
+
cells. CD8a is further detected on most thymocytes (CD4
+
CD8a
+
and CD4
CD8
+
thymocytes). Cytotoxic T cells and thymocytes express CD8a as heterodimer with CD8b, whereas dendritic cells and TCRγ/δ
+
cells express a CD8a/CD8a homodimer. It mediates efficient cell-cell interactions within the immune system.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

CD8, Leu-2, Ly-2, T8, Lyt-2, Ly-35

Technical specifications

  • Antigen: CD8a
  • Clone: REAL137
  • Isotype control: REAL Control
  • Alternative names of antigen: CD8, Leu-2, Ly-2, T8, Lyt-2, Ly-35
  • Distribution of antigen: T cells, dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, VioBlue, VioGreen, FITC
  • Selected references

    1. Nakauchi, H. et al. (1985) Molecular cloning of Lyt-2, a membrane glycoprotein marking a subset of mouse T lymphocytes: molecular homology to its human counterpart, Leu-2/T8, and to immunoglobulin variable regions. Proc. Natl. Acad. Sci. U.S.A. 82(15): 5126-5130
    2. Liaw, C. W. et al. (1986) Structure, sequence, and polymorphism of the Lyt-2 T cell differentiation antigen gene. J. Immunol. 137(3): 1037-1043
    3. Nakauchi, H. et al. (1987) Isolation and characterization of the gene for the murine T cell differentiation antigen and immunoglobulin-related molecule, Lyt-2. Nucleic Acids Res. 15(10): 4337-4347
Product options: 8
130-112-082

REAlease CD8a-FITC, mouse

30 µg in 200 µL (1)
-
130-118-065

REAlease CD8a-PE, mouse

30 µg in 200 µL (1)
-
130-119-604

REAlease CD8a-VioBlue, mouse

30 µg in 200 µL (1)
-
130-115-971

REAlease CD8a-VioGreen, mouse

30 µg in 200 µL (1)
-
130-122-720

REAlease CD8a-FITC Kit, mouse

for 100
tests
-
130-122-721

REAlease CD8a-PE Kit, mouse

for 100
tests
-
130-122-722

REAlease CD8a-VioBlue Kit, mouse

for 100
tests
-
130-122-723

REAlease CD8a-VioGreen Kit, mouse

for 100
tests
-