REAlease® CD49b Complexes, mouse

CD49b Complexes
, mouse

Clone: REAL133 | Dilution: 1:50
Clone REAL133 is an antibody fragment derived from the full CD49b antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL133 recognizes the mouse CD49b antigen, a single-pass type I membrane protein also known as integrin alpha-2 (ITGA2). CD49b together with CD29 (ITGB1) forms the adhesion molecule very late antigen-2 (VLA-2), which binds mainly to collagens I, II, and XI. VLA-2 is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation, and organization of newly synthesized extracellular matrix. It is also a receptor for laminins, collagen C-propeptides, and E-cadherin. Mice homozygous for a null mutation in the CD49b gene die very early in embryogenesis. CD49b is expressed on the vast majority of mouse natural killer (NK) cells and on a subset of NKT cells. It is not as mouse strain-restricted as other NK cell markers, for example, NK1.1, and is expressed by most common inbred mouse strains. Bone marrow (BM) resident memory T helper (Tʜ) cells also express CD49b and the homing of adoptively transferred bone marrow (BM) memory Tʜ cells to BM can be efficiently blocked by antibodies to CD49b. This points to a decisive role of CD49b in the trafficking of memory Th cell precursors to the BM niches for memory Tʜ cells.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

Itga2, gpIa, VLA-2α, VLA-2alpha, DX5

Technical specifications

  • Antigen: CD49b
  • Clone: REAL133
  • Isotype control: REAL Control
  • Alternative names of antigen: Itga2, gpIa, VLA-2α, VLA-2alpha, DX5
  • Distribution of antigen: NK cells, NKT cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC
  • Selected references

    1. Edelman, J. M. et al. (1994) The mouse VLA-2 homologue supports collagen and laminin adhesion but not virus binding. Cell Adhes. Commun. 2(2): 131-143
    2. Gagliani, N. et al. (2013) Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells. Nat. Med. 19(6): 739-746
    3. Hanazawa, A. et al. (2013) CD49b/CD69-dependent generation of resting T helper cell memory. Front Immunol 4: 183
Product options: 6

REAlease CD49b-FITC, mouse

30 µg in 200 µL (1)

REAlease CD49b-PE, mouse

30 µg in 200 µL (1)

REAlease CD49b-APC, mouse

30 µg in 200 µL (1)

REAlease CD49b-FITC Kit, mouse

for 100

REAlease CD49b-PE Kit, mouse

for 100

REAlease CD49b-APC Kit, mouse

for 100