CD95 (FAS) antibodies, mouse

CD95 (FAS) antibodies, mouse

Clone: REA453 | Dilution: 1:50
Clone REA453 recognizes the mouse CD95 antigen, a single-pass type I membrane protein which is also known as FAS or Apo-1. CD95 is a member of the tumor necrosis factor receptor superfamily and is found on the surface of many normal and neoplastically transformed cells. It is an apoptosis-inducing death receptor that regulates tissue homeostasis mainly in the immune system through induction of apoptosis by binding of its ligand CD95L (FASL/Apo-1L). CD95 and CD95L are up-regulated on lymphocytes upon activation and are known to play a key role in the regulation of an inflammatory response. CD95 also plays an apoptosis-independent role in non-immune cells and it has been implicated in cancer cell growth, migration, and tumor progression. During cancer progression CD95 is frequently down regulated or cells are rendered apoptosis resistant raising the possibility that loss of CD95 is part of a mechanism for tumor evasion.
Additional information: Clone REA453 displays negligible binding to Fc receptors.

Alternative names

FAS, APO-1, Apt1, Tnfrsf6, TNFR6, lpr

Technical specifications

  • Antigen: CD95 (FAS)
  • Clone: REA453
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: FAS, APO-1, Apt1, Tnfrsf6, TNFR6, lpr
  • Entrez Gene ID: 14102
  • Molecular mass of antigen [kDa]: 35
  • Distribution of antigen: lymphocytes, other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE

Specificity

In order to compare the epitope specificity of REAfinity Clone REA453 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA453
Jo2++
SA367H8-
  • Selected references

    1. Watanabe-Fukunaga, R. et al. (1992) The cDNA structure, expression, and chromosomal assignment of the mouse Fas antigen. J. Immunol. 148(4): 1274-1279
    2. Chen, L. et al. (2010) CD95 promotes tumour growth. Nature 465(7297): 492-496
    3. Hadji, A. et al. (2014) Death induced by CD95 or CD95 ligand elimination. Cell Rep 7(1): 208-222
Product options: 2
130-119-657

CD95 (FAS)-PE, mouse

30 µg in 200 µL (1)
-
130-119-557

CD95 (FAS)-PE, mouse

150 µg in 1 mL (1)
-