CD8a antibodies, rat

CD8a antibodies, rat

Clone: REA437 | Dilution: 1:50
Clone REA437 recognizes the rat CD8a antigen, a single-pass type I membrane protein, which is also known as OX-8. CD8 acts as a co-receptor for the T cell receptor and recognizes antigen displayed by an antigen presenting cell in the context of class I MHC molecules. CD8a is expressed as a heterodimer with CD8b on T cell receptor (TCR) α/β and TCR γ/δ expressing, cytotoxic T cells, and on most thymocytes. As a homodimer of two CD8a chains the CD8 antigen is expressed on most NK cells, a major fraction of intestinal intraepithelial lymphocytes, some activated CD4
+
CD8
+
T cells and CD8
+
T cells from athymic rats. CD8a is also expressed by a T cell subset with regulatory functions.
Additional information: Clone REA437 displays negligible binding to Fc receptors.

Alternative Names

OX-8, Lyt2, Ly-2

Technical specifications

  • Antigen: CD8a
  • Clone: REA437
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: OX-8, Lyt2, Ly-2
  • Entrez Gene ID: 24930
  • Molecular mass of antigen [kDa]: 23
  • Distribution of antigen: NK cells, T cells, thymocytes, other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC
  • Selected References

    1. Johnson, P. et al. (1985) Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes. EMBO J. 4(10): 2539-2545
    2. Torres-Nagel, N. et al. (1992) Differential thymus dependence of rat CD8 isoform expression. Eur. J. Immunol. 22(11): 2841-2848
    3. Strup-Perrot, C. et al. (2005) Expression of matrix metalloproteinases and tissue inhibitor metalloproteinases increases in X-irradiated rat ileum despite the disappearance of CD8a T cells. World J Gastroenterol. 11(40): 6312-6321