CD8a antibodies, mouse

CD8a antibodies, mouse

Clone: REA601 | Dilution: 1:10
Clone REA601 is specific for the mouse CD8a antigen, also known as LY-2 or T8, which is expressed on cytotoxic T cells and at lower levels on subpopulations of dendritic cells and TCRγ/δ
+
cells. CD8a is further detected on most thymocytes (CD4
+
CD8a
+
and CD4
CD8
+
thymocytes). Cytotoxic T cells and thymocytes express CD8a as heterodimer with CD8b, whereas dendritic cells and TCRγ/δ
+
cells express a CD8a/CD8a homodimer. It mediates efficient cell-cell interactions within the immune system.
Additional information: Clone REA601 displays negligible binding to Fc receptors.

Alternative Names

CD8, Leu-2, Ly-2, T8, Lyt-2

Technical specifications

  • Antigen: CD8a
  • Clone: REA601
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: CD8, Leu-2, Ly-2, T8, Lyt-2
  • Entrez Gene ID: 12525
  • Molecular mass of antigen [kDa]: 25
  • Distribution of antigen: dendritic cells, NK cells, red blood cells, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, VioGreen, PE-Vio615, APC-Vio770

Specificity

In order to compare the epitope specificity of REAfinity Clone REA601 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA601
53-6.7++
Product options: 10
130-109-556

CD8a-VioBright FITC, mouse

9 µg in 300 µL (1)
-
130-109-477

CD8a-VioBright FITC, mouse

30 µg in 1 mL (1)
-
130-109-325

CD8a-PE, mouse

9 µg in 300 µL (1)
-
130-109-247

CD8a-PE, mouse

30 µg in 1 mL (1)
-
130-109-330

CD8a-VioGreen, mouse

9 µg in 300 µL (1)
-
130-109-252

CD8a-VioGreen, mouse

30 µg in 1 mL (1)
-
130-109-329

CD8a-PE-Vio615, mouse

9 µg in 300 µL (1)
-
130-109-251

CD8a-PE-Vio615, mouse

30 µg in 1 mL (1)
-
130-109-328

CD8a-APC-Vio770, mouse

9 µg in 300 µL (1)
-
130-109-250

CD8a-APC-Vio770, mouse

30 µg in 1 mL (1)
-