CD66b antibodies, human

CD66b antibodies, human

Clone: REA306
Clone REA306 recognizes the human CD66b antigen, a glycosylphosphatidylinositol (GPI)–linked protein, which is also known as carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8). CD66b is exclusively expressed on neutrophils and eosinophils and on leukocytes of chronic myeloid leukemia patients and bone marrow. Under normal conditions, neutrophils have minimal expression of CD66b, but incubation
in vitro
with inflammatory agonists rapidly increases this expression. Furthermore, expression of CD66b in eosinophils is enhanced
in vivo
in patients with a helminth infection, specifically with
Schistosoma mansoni
. The eosinophil expression of CD66b is also enhanced
in vitro
by incubation with activated lymphocytes or by brief incubation with interleukin 5 (IL-5). Crosslinking of CD66b on peripheral blood neutrophils mediates the release of IL-8 from intracellular storage.
Additional information: Clone REA306 displays negligible binding to Fc receptors.

Alternative Names

CEACAM8, CD67, CGM6, NCA-95
REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the different hybridoma clones. 
Other clonesOverlap in epitope recognition with REA306
G10F5++
80H3-

Technical specifications

  • Antigen: CD66b
  • Clone: REA306
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: CEACAM8, CD67, CGM6, NCA-95
  • Entrez Gene ID: 1088
  • Molecular mass of antigen [kDa]: 32
  • Distribution of antigen: eosinophils, neutrophils, bone marrow, other
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
Product options: -

Product information

Size
Order no.
Price