CD66b antibodies, human

CD66b antibodies, human

Clone: REA306
Clone REA306 recognizes the human CD66b antigen, a glycosylphosphatidylinositol (GPI)–linked protein, which is also known as carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8). CD66b is exclusively expressed on neutrophils and eosinophils and on leukocytes of chronic myeloid leukemia patients and bone marrow. Under normal conditions, neutrophils have minimal expression of CD66b, but incubation
in vitro
with inflammatory agonists rapidly increases this expression. Furthermore, expression of CD66b in eosinophils is enhanced
in vivo
in patients with a helminth infection, specifically with
Schistosoma mansoni
. The eosinophil expression of CD66b is also enhanced
in vitro
by incubation with activated lymphocytes or by brief incubation with interleukin 5 (IL-5). Crosslinking of CD66b on peripheral blood neutrophils mediates the release of IL-8 from intracellular storage.
Additional information: Clone REA306 displays negligible binding to Fc receptors.

Alternative Names

CEACAM8, CD67, CGM6, NCA-95

Technical specifications

  • Antigen: CD66b
  • Clone: REA306
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: CEACAM8, CD67, CGM6, NCA-95
  • Entrez Gene ID: 1088
  • Molecular mass of antigen [kDa]: 32
  • Distribution of antigen: eosinophils, neutrophils, bone marrow, other
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure

Specificity

In order to compare the epitope specificity of REAfinity Clone REA306 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA306
G10F5++
80H3-
  • Selected references

    1. Berling, B. et al. (1990) Cloning of a carcinoembryonic antigen gene family member expressed in leukocytes of chronic myeloid leukemia patients and bone marrow. Cancer Res. 50(20): 6534-6539
    2. Eades-Perner, A. M. et al. (1998) Mice transgenic for the human CGM6 gene express its product, the granulocyte marker CD66b, exclusively in granulocytes. Blood 91(2): 663-672
    3. Yoon, J. et al. (2007) CD66b regulates adhesion and activation of human eosinophils. J. Immunol. 179(12): 8454-8462
Product options: 1
130-108-019

CD66b pure, human

100 µg in 100 µL
-