CD62L antibodies, mouse

CD62L antibodies, mouse

Clone: REA828 | Dilution: 1:50
Clone REA828 recognizes mouse CD62L, also known as L-selectin. CD62L is a member of the selectin family of cell adhesion molecules. CD62L is important for the homing of lymphocytes to peripheral lymph nodes through high endothelial venules. It also contributes to leukocyte emigration into acute inflammatory sites. CD62L is a glycoprotein expressed on the cell surface of most thymocytes, T cells, B cells, monocytes, dendritic cells, neutrophils, and eosinophils. It is highly expressed on naive T cells and down-regulated upon differentiation due to activation-dependent shedding. CD62L is also expressed on a subset of CCR7
high
CD44
high
central memory T cells and on CD4
+
CD25
+
regulatory T cells.
Additional information: Clone REA828 displays negligible binding to Fc receptors.

Alternative Names

Sell, L-selectin, LECAM-1, Lnhr, Ly-22, Ly-m22, Lyam-1, Lyam1, LAM-1

Technical specifications

  • Antigen: CD62L
  • Clone: REA828
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: Sell, L-selectin, LECAM-1, Lnhr, Ly-22, Ly-m22, Lyam-1, Lyam1, LAM-1
  • Entrez Gene ID: 20343
  • Molecular mass of antigen [kDa]: 38
  • Distribution of antigen: B cells, bone marrow, eosinophils, granulocytes, Langerhans cells, lymphocytes, monocytes, neutrophils, NK cells, red blood cells, spleen, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA828
MEL14++
95218-
  • Selected References

    1. Siegelman, M. H. et al. (1990) The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding. Cell 61(4): 611-622
    2. Gallatin, W. M. et al. (2006) A cell-surface molecule involved in organ-specific homing of lymphocytes. J. Immunol. 177(1): 5-9
Product options: 18

Product information

Size
Order no.
Price

CD62L-FITC, mouse

  • REA828
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-835
-

CD62L-FITC, mouse

  • REA828
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-646
-

CD62L-PE, mouse

  • REA828
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-836
-

CD62L-PE, mouse

  • REA828
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-647
-

CD62L-APC, mouse

  • REA828
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-837
-

CD62L-APC, mouse

  • REA828
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-648
-

CD62L-VioBlue, mouse

  • REA828
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-841
-

CD62L-VioBlue, mouse

  • REA828
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-652
-

CD62L-PE-Vio615, mouse

  • REA828
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-842
-

CD62L-PE-Vio615, mouse

  • REA828
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-653
-

CD62L-PE-Vio770, mouse

  • REA828
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-838
-

CD62L-PE-Vio770, mouse

  • REA828
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-649
-

CD62L-APC-Vio770, mouse

  • REA828
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-839
-

CD62L-APC-Vio770, mouse

  • REA828
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-650
-

CD62L-PerCP-Vio700, mouse

  • REA828
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-840
-

CD62L-PerCP-Vio700, mouse

  • REA828
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-651
-

CD62L-Biotin, mouse

  • REA828
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-834
-

CD62L-Biotin, mouse

  • REA828
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-645
-