CD62L antibodies, human

CD62L antibodies, human

Clone: REA615 | Dilution: 1:11
Clone REA615 recognizes the human CD62L antigen, a 74 kDa single-pass type I membrane protein, which is also known as L-selectin, LECAM-1, or LAM-1. CD62L is a member of the selectin family of cell surface molecules and binds a series of glycoproteins including CD34, GlyCAM-1, and MAdCAM-1. Most hematopoietic cells express CD62L, including peripheral blood B cells, T cells, monocytes, granulocytes, thymocytes, and some myeloid cells from bone marrow. Expression on resting naive, central memory, and some effector memory T cells regulates their migration via endothelial venules to peripheral lymph nodes and Peyer’s patches. The CD62L antigen also contributes to the recruitment of leukocytes from the blood to areas of inflammation. Disruption of CD62L expression has detrimental effects on T cell migration and immune responses. Antigenic activation in the lymph node leads first to rapid CD62L shedding by protease cleavage and then to transcriptional suppression.
Always use fresh material for immunofluorescent staining of CD62L
+
cells. For optimal results, the cells should not be older than 8–12 hours. Keep cells continuously cold. CD62L-expression may be rapidly lost due to shedding.
Additional information: Clone REA615 displays negligible binding to Fc receptors.

Alternative Names

L-selectin, LAM-1, LECAM-1, Leu-8, TQ1, gp90-MEL

Technical specifications

  • Antigen: CD62L
  • Clone: REA615
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: L-selectin, LAM-1, LECAM-1, Leu-8, TQ1, gp90-MEL
  • Entrez Gene ID: 6402
  • Molecular mass of antigen [kDa]: 38
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
    , baboon
  • Distribution of antigen: B cells, bone marrow, eosinophils, granulocytes, Langerhans cells, lymphocytes, monocytes, neutrophils, NK cells, red blood cells, spleen, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, Biotin
Product options: 2

Product information

Size
Order no.
Price

CD62L-Biotin, human

  • REA615
  • 1:11
  • For research use only
for 30
tests
(1)
130-109-554
-

CD62L-Biotin, human

  • REA615
  • 1:11
  • For research use only
for 100
tests
(1)
130-109-475
-

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