CD59 antibodies, rat

CD59 antibodies, rat

Clone: REA452 | Dilution: 1:10
Clone REA452 recognizes the rat CD59 antigen, a 21 kDa glycosylphosphatidylinositol (GPI) linked cell-surface glycoprotein which is also known as MAC-inhibitory protein (MAC-IP), membrane attack complex inhibition factor (MACIF), or protectin. CD59 is widely distributed, being present on all epithelia, endothelia, and on circulating cells, including erythrocytes, monocytes, and some lymphocytes. It inhibits the lytic activity of complement by binding to C8 and C9, thereby blocking the incorporation of mutiple C9 molecules into the membrane attack complex thus preventing functional pore formation.
Additional information: Clone REA452 displays negligible binding to Fc receptors.

Alternative Names

CD59a, CD59b, MACIF, MAC-IP, Protectin

Technical specifications

  • Antigen: CD59
  • Clone: REA452
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: CD59a, CD59b, MACIF, MAC-IP, Protectin
  • Entrez Gene ID: 25407
  • Molecular mass of antigen [kDa]: 9
  • Distribution of antigen: epithelial cells, endothelial cells, monocytes, lymphocytes, other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, PE-Vio770, APC-Vio770, Biotin
  • Selected References

    1. Rushmere, N. K. et al. (1994) Molecular cloning of the rat analogue of human CD59: structural comparison with human CD59 and identification of a putative active site. Biochem. J. 304(Pt 2): 595-601
    2. Rushmere, N. K. et al. (1997) Expression of rat CD59: functional analysis confirms lack of species selectivity and reveals that glycosylation is not required for function. Immunology 90(4): 640-646
    3. Mizuno, T. et al. (2011) Specific collaboration between rat membrane complement regulators Crry and CD59 protects peritoneum from damage by autologous complement activation. Nephrol. Dial. Transplant. 26(6): 1821-1830
Product options: 12
130-106-864

CD59-VioBright FITC, rat

9 µg in 300 µL (1)
-
130-106-820

CD59-VioBright FITC, rat

30 µg in 1 mL (1)
-
130-106-860

CD59-PE, rat

9 µg in 300 µL (1)
-
130-106-816

CD59-PE, rat

30 µg in 1 mL (1)
-
130-106-861

CD59-APC, rat

9 µg in 300 µL (1)
-
130-106-817

CD59-APC, rat

30 µg in 1 mL (1)
-
130-106-862

CD59-PE-Vio770, rat

9 µg in 300 µL (1)
-
130-106-818

CD59-PE-Vio770, rat

30 µg in 1 mL (1)
-
130-106-863

CD59-APC-Vio770, rat

9 µg in 300 µL (1)
-
130-106-819

CD59-APC-Vio770, rat

30 µg in 1 mL (1)
-
130-106-859

CD59-Biotin, rat

9 µg in 300 µL (1)
-
130-106-815

CD59-Biotin, rat

30 µg in 1 mL (1)
-