CD56 antibodies, human

CD56 antibodies, human

Clone: REA196 | Dilution: 1:50
Clone REA196 recognizes the human CD56 antigen, a glycoprotein of the Ig-superfamily, also known as neural cell adhesion molecule (NCAM), which is expressed in blood on practically all resting and activated NK cells and on a minor subset of CD3
+
T cells. CD56 is also expressed in brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal-derived tumors, myelomas, and myeloid leukemias also express CD56.
Additional information: Clone REA196 displays negligible binding to Fc receptors.

Alternative Names

NCAM1, MSK39, NCAM

Technical specifications

  • Antigen: CD56
  • Clone: REA196
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: NCAM1, MSK39, NCAM
  • Entrez Gene ID: 4684
  • Molecular mass of antigen [kDa]: 92
  • Cross-reactivity:
    chimpanzee (
    Pan troglodytes
    )
    ,
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
    ,
    olive baboon (
    Papio anubis
    )
  • Distribution of antigen: NK cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, VioBright 515, Biotin
  • Selected References

    1. Camp, B. V. et al. (1990) Plasma cells in multiple myeloma express a natural killer cell- associated antigen: CD56 (NKH-1; Leu-19). Blood 76: 377-382
    2. Popkin, D. L. et al. (2018) In vitro Evidence That Combination Therapy With CD16-Bearing NK-92 Cells and FDA-Approved Alefacept Can Selectively Target the Latent HIV Reservoir in CD4+ CD2hi Memory T Cells. Front Immunol 9: 2552
    3. Grant, M. D. et al. (2018) Natural Killer Cells Adapt to Cytomegalovirus Along a Functionally Static Phenotypic Spectrum in Human Immunodeficiency Virus Infection. Front Immunol 9: 2494
    4. Delso-Vallejo, M. et al. (2017) Influence of Irradiated Peripheral Blood Mononuclear Cells on Both Ex Vivo Proliferation of Human Natural Killer Cells and Change in Cellular Property. Front Immunol 8
    5. Croci, S. et al. (2018) Higher Frequencies of Lymphocytes Expressing the Natural Killer Group 2D Receptor in Patients With Behçet Disease. Front Immunol 9: 2157
    6. Canté-Barrett, K. et al. (2017)
      Loss of CD44
      dim
      expression from early progenitor cells marks T-cell lineage commitment in the human thymus.
      Front Immunol 8: 32
    7. Dong, P. et al. (2017) Simultaneous detection of decidual Th1/Th2 and NK1/NK2 immunophenotyping in unknown recurrent miscarriage using 8-color flow cytometry with FSC/Vt extended strategy. Biosci. Rep. 37(3)
    8. Petty, R. D. et al. (2016) MicroRNAs hsa-miR-99b, hsa-miR-330, hsa-miR-126 and hsa-miR-30c: potential diagnostic biomarkers in natural killer (NK) cells of patients with chronic fatigue syndrome (CFS)/ myalgic encephalomyelitis (ME). PLoS One 11: e0150904
    9. Boin, F. et al. (2017) Flow cytometric discrimination of seven lineage markers by using two fluorochromes. PLoS One 12(11)
    10. Vivier, E. et al. (2018) Anti-NKG2A mAb Is a Checkpoint Inhibitor that Promotes Anti-tumor Immunity by Unleashing Both T and NK Cells. Cell 175(7): 1731-1743
    11. Veluchamy, J. et al. (2017)
      In vivo
      efficacy of umbilical cord blood stem cell-derived NK cells in the treatment of metastatic colorectal cancer.
      Front Immunol 8: 87
REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 
Other clones Overlap in epitope recognition with REA196
NCAM16.2 ++
TULY56 ++
5.1H11 ++
AF12-7H3 -
C5.9 ++
N901/NKH1 ++
CMSSB +
B159

 -

HCD56-
MEM188-
Product options: 18
130-114-740

CD56-FITC, human

for 30
tests
(1)
-
130-114-549

CD56-FITC, human

for 100
tests
(1)
-
130-113-874

CD56-PE, human

for 30
tests
(1)
-
130-113-312

CD56-PE, human

for 100
tests
(1)
-
130-113-872

CD56-APC, human

for 30
tests
(1)
-
130-113-310

CD56-APC, human

for 100
tests
(1)
-
130-114-741

CD56-PE-Vio615, human

for 30
tests
(1)
-
130-114-550

CD56-PE-Vio615, human

for 100
tests
(1)
-
130-113-875

CD56-PE-Vio770, human

for 30
tests
(1)
-
130-113-313

CD56-PE-Vio770, human

for 100
tests
(1)
-
130-114-739

CD56-APC-Vio770, human

for 30
tests
(1)
-
130-114-548

CD56-APC-Vio770, human

for 100
tests
(1)
-
130-114-742

CD56-PerCP-Vio700, human

for 30
tests
(1)
-
130-114-551

CD56-PerCP-Vio700, human

for 100
tests
(1)
-
130-114-743

CD56-VioBright 515, human

for 30
tests
(1)
-
130-114-552

CD56-VioBright 515, human

for 100
tests
(1)
-
130-113-873

CD56-Biotin, human

for 30
tests
(1)
-
130-113-311

CD56-Biotin, human

for 100
tests
(1)
-