CD53 antibodies, human

CD53 antibodies, human

Clone: REA259 | Dilution: 1:11
Clone REA259 recognizes the CD53 antigen, a 35–42 kDa type II transmembrane glycoprotein, which is also known as tetraspanin-25. CD53 is expressed on all leukocytes including plasma cells, but not on platelets, erythrocytes, and non-hematopoietic tissues. CD53 mediates oxidative burst in monocytes and has been shown to interact with a number of proteins including IL-4, CD20, CD2, CD9, IL-2, VLA-4, and CD4. Cross-linking of CD53 on monocytes induces a strong increase in intracellular calcium ions, whereas a modest increase is seen in B lymphocytes. In resting B cells, the molecule transmits a co-stimulatory signal and promotes activation through the Ig receptor.
Additional information: Clone REA259 displays negligible binding to Fc receptors.

Alternative Names

MOX44, TSPAN25

Technical specifications

  • Antigen: CD53
  • Clone: REA259
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: MOX44, TSPAN25
  • Distribution of antigen: B cells, T cells, monocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, APC-Vio770, Biotin
  • Selected References

    1. Olweus, J. et al. (1993) CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J. Immunol. 151: 707-716
    2. Maecker, H. T, et al. (1997) The tetraspanin superfamily: molecular facilitators. FASEB J. 11: 428-442
    3. Angelisova, P. et al. (1990) The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens. Immunogenetics 32(4): 281-285
Product options: 12

Product information

Size
Order no.
Price

CD53-FITC, human

  • REA259
  • 1:11
  • For research use only
for 30
tests
(1)
130-101-798
-

CD53-FITC, human

  • REA259
  • 1:11
  • For research use only
for 100
tests
(1)
130-101-808
-

CD53-PE, human

  • REA259
  • 1:11
  • For research use only
for 30
tests
(1)
130-101-799
-

CD53-PE, human

  • REA259
  • 1:11
  • For research use only
for 100
tests
(1)
130-101-782
-

CD53-APC, human

  • REA259
  • 1:11
  • For research use only
for 30
tests
(1)
130-101-795
-

CD53-APC, human

  • REA259
  • 1:11
  • For research use only
for 100
tests
(1)
130-101-780
-

CD53-PE-Vio770, human

  • REA259
  • 1:11
  • For research use only
for 30
tests
(1)
130-101-800
-

CD53-PE-Vio770, human

  • REA259
  • 1:11
  • For research use only
for 100
tests
(1)
130-101-769
-

CD53-APC-Vio770, human

  • REA259
  • 1:11
  • For research use only
for 30
tests
(1)
130-101-797
-

CD53-APC-Vio770, human

  • REA259
  • 1:11
  • For research use only
for 100
tests
(1)
130-101-781
-

CD53-Biotin, human

  • REA259
  • 1:11
  • For research use only
for 30
tests
(1)
130-101-817
-

CD53-Biotin, human

  • REA259
  • 1:11
  • For research use only
for 100
tests
(1)
130-101-814
-