CD52 antibodies, human

CD52 antibodies, human

Clone: REA164 | Dilution: 1:11
Clone REA164 recognizes CD52, a glycosylphosphatidylinositol (GPI)-anchored, 25–29 kDa heavily glycosylated antigen. The CD52 core peptide is only 12 amino acids long and is linked to the surface via C-terminal GPI anchor. Expression of CD52 is found on lymphocytes, monocytes, macrophages, eosinophils, and in the male reproductive tract on the epithelial cells of the epididymis and seminal vesicle. CD52 is absent in immature spermatozoa in the efferent ducts but is acquired by the sperm membrane, progressively, as they pass through the epididymis proper, where CD52 is shed into the seminal plasma by the epididymis. Though the exact role of CD52 is unclear, antibodies against CD52 elicit antibody dependent cellular and complement mediated cytolysis of the CD52 expressing lymphocytes. Owing to this, such antibodies are often used to target malignant cells. CD52 expressed on sperms and on lymphocytes are basically different glycoforms of the same core peptide.
Additional information: Clone REA164 displays negligible binding to Fc receptors.

Alternative names

CDW52, CAMPATH-1

Technical specifications

  • Antigen: CD52
  • Clone: REA164
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: CDW52, CAMPATH-1
  • Entrez Gene ID: 1043
  • Molecular mass of antigen [kDa]: 1
  • Distribution of antigen: lymphocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, PE-Vio770, APC-Vio770, Biotin
  • Selected references

    1. Ambrose, L. R. et al. (2009) Neutrophils express CD52 and exhibit complement-mediated lysis in the presence of alemtuzumab. Blood 114: 3052-3055
    2. Rawstron, A. C. et al. (2006) CD52 expression patterns in myeloma and the applicability of alemtuzumab therapy. Haematologica 91: 1577-1578
    3. Yeung, C. H. et al. (1998) Epididymal secretion of CD52 as measured in human seminal plasma by a fluorescence immunoassay. Mol. Hum. Reprod. 4: 447-451
Product options: 14
130-099-641

CD52-FITC, human

for 30
tests
(1)
-
130-099-643

CD52-FITC, human

for 100
tests
(1)
-
130-099-634

CD52-PE, human

for 30
tests
(1)
-
130-099-635

CD52-PE, human

for 100
tests
(1)
-
130-099-630

CD52-APC, human

for 30
tests
(1)
-
130-099-632

CD52-APC, human

for 100
tests
(1)
-
130-099-626

CD52-VioBlue, human

for 30
tests
(1)
-
130-099-628

CD52-VioBlue, human

for 100
tests
(1)
-
130-099-622

CD52-PE-Vio770, human

for 30
tests
(1)
-
130-099-624

CD52-PE-Vio770, human

for 100
tests
(1)
-
130-099-617

CD52-APC-Vio770, human

for 30
tests
(1)
-
130-099-619

CD52-APC-Vio770, human

for 100
tests
(1)
-
130-099-645

CD52-Biotin, human

for 30
tests
(1)
-
130-099-648

CD52-Biotin, human

for 100
tests
(1)
-