CD49f antibodies, human and mouse

CD49f antibodies, human and mouse

Clone: GoH3 | Dilution: 1:11
Clone GoH3 reacts with CD49f, a 120 kDa transmembrane glycoprotein also known as α6 integrin. Integrins are integral cell-surface proteins composed of an α chain and a β chain and are involved in cell adhesion as well as cell-surface mediated signaling. CD49f associates with integrin β1 chain (CD29) to form VLA-6 and with integrin β4 chain (CD104) to form the α6 β4 complex, which plays a critical structural role in the hemidesmosome and constitutes a receptor for laminin in epithelial cells. CD49f is furthermore expressed on T cells, monocytes, platelets, endothelial cells, trophoblasts of placenta, and spermatogonial stem cells. Clone GoH3 antibody has been reported to block laminin binding
in vitro
and to block integrin α6 function
in vivo
.

Alternative Names

ITGA6, α6 integrin, ITGA6B, VLA-6, CD49f, VLA-6α

Technical specifications

  • Antigen: CD49f
  • Clone: GoH3
  • Isotype: rat IgG2aκ
  • Isotype control: Rat IgG2a – isotype control antibodies
  • Alternative names of antigen: ITGA6, α6 integrin, ITGA6B, VLA-6, CD49f, VLA-6α
  • Entrez Gene ID: 3655
  • Molecular mass of antigen [kDa]: 124
  • Cross-reactivity: porcine, dog,
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
    ,
    olive baboon (
    Papio anubis
    )
    ,
    chimpanzee (
    Pan troglodytes
    )
    , capuchin monkey
  • Distribution of antigen: brain, cancer stem cells, epithelial cells, hematopoietic stem cells, kidney, liver, lung, lymphocytes, megakaryocytes, monocytes, ovary, platelets, ES and iPS cells, spleen, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, Biotin
  • Selected References

    1. Hynes, R. O. (2002) Integrins: bidirectional, allosteric signaling machines. Cell 110(6): 673-687
    2. Aumailley, M. et al. (1990) Antibody to integrin α 6 subunit specifically inhibits cell-binding to laminin fragment 8. Exp. Cell Res. 188(1): 55-60
    3. Hemler, M. E. et al. (1988) Multiple very late antigen (VLA) heterodimers on platelets. Evidence for distinct VLA-2, VLA-5 (fibronectin receptor), and VLA-6 structures. J. Biol. Chem. 263: 7660-7665
    4. Carter, W. G. et al. (1990) Distinct functions for integrins α 3 β 1 in focal adhesions and α 6 β 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes. J. Cell Biol. 111: 3141-3154
    5. Izadyar, F. et al. (2011) Identification and characterization of repopulating spermatogonial stem Hum. Reprod. 26: 1296-1306
Product options: 6

Product information

Size
Order no.
Price

CD49f-FITC, human and mouse

  • GoH3
  • 1:11
  • For research use only
for 100
tests
(1)
130-097-245
-

CD49f-PE, human and mouse

  • GoH3
  • 1:11
  • For research use only
for 30
tests
(1)
130-100-096
-

CD49f-PE, human and mouse

  • GoH3
  • 1:11
  • For research use only
for 100
tests
(1)
130-097-246
-

CD49f-APC, human and mouse

  • GoH3
  • 1:11
  • For research use only
for 30
tests
(1)
130-100-147
-

CD49f-APC, human and mouse

  • GoH3
  • 1:11
  • For research use only
for 100
tests
(1)
130-097-250
-

CD49f-Biotin, human and mouse

  • GoH3
  • 1:11
  • For research use only
for 100
tests
(1)
130-097-243
-