CD45RO antibodies, human

CD45RO antibodies, human

Clone: REA611 | Dilution: 1:50
Clone REA611 recognizes the human CD45RO antigen, a 180 kDa single-chain membrane glycoprotein, which is an isoform of CD45. CD45RO is expressed by most thymocytes, activated and memory T cells, granulocytes, B cell subsets, and monocytes. CD45RO binds to galectin-1 and CD22. CD45RO antibodies are commonly used in combination with antibodies against CD45RA to discriminate memory T cells from naive T cells.
Additional information: Clone REA611 displays negligible binding to Fc receptors.

Technical specifications

  • Antigen: CD45RO
  • Clone: REA611
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Entrez Gene ID: 5788
  • Molecular mass of antigen [kDa]: 145
  • Cross-reactivity:
    chimpanzee (
    Pan troglodytes
    )
    ,
    common marmoset (
    Callithrix jacchus
    )
  • Distribution of antigen: thymocytes, granulocytes, T cells, B cells, monocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, VioBlue

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA611
UCHL1++
  • Selected References

    1. Charbonneau, H. et al. (1988) The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase. Proc. Natl. Acad. Sci. U.S.A. 85(19): 7182-7186
    2. Trowbridge, I. S. et al. (1994) CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. Annu. Rev. Immunol. 12: 85-116
Product options: 16
130-114-084

CD45RO-FITC, human

for 30
tests
(1)
-
130-113-558

CD45RO-FITC, human

for 100
tests
(1)
-
130-114-085

CD45RO-PE, human

for 30
tests
(1)
-
130-113-559

CD45RO-PE, human

for 100
tests
(1)
-
130-114-082

CD45RO-APC, human

for 30
tests
(1)
-
130-113-556

CD45RO-APC, human

for 100
tests
(1)
-
130-114-087

CD45RO-VioGreen, human

for 30
tests
(1)
-
130-113-561

CD45RO-VioGreen, human

for 100
tests
(1)
-
130-114-088

CD45RO-PE-Vio615, human

for 30
tests
(1)
-
130-113-562

CD45RO-PE-Vio615, human

for 100
tests
(1)
-
130-114-086

CD45RO-PE-Vio770, human

for 30
tests
(1)
-
130-113-560

CD45RO-PE-Vio770, human

for 100
tests
(1)
-
130-114-083

CD45RO-APC-Vio770, human

for 30
tests
(1)
-
130-113-557

CD45RO-APC-Vio770, human

for 100
tests
(1)
-
130-119-710

CD45RO-VioBlue, human

for 30
tests
(1)
-
130-119-620

CD45RO-VioBlue, human

for 100
tests
(1)
-