CD45RO antibodies, human

CD45RO antibodies, human

Clone: REA611 | Dilution: 1:50
Clone REA611 recognizes the human CD45RO antigen, a 180 kDa single-chain membrane glycoprotein, which is an isoform of CD45. CD45RO is expressed by most thymocytes, activated and memory T cells, granulocytes, B cell subsets, and monocytes. CD45RO binds to galectin-1 and CD22. CD45RO antibodies are commonly used in combination with antibodies against CD45RA to discriminate memory T cells from naive T cells.
Additional information: Clone REA611 displays negligible binding to Fc receptors.
REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the different hybridoma clones. 
Other clonesOverlap in epitope recognition with REA611

Technical specifications

  • Antigen: CD45RO
  • Clone: REA611
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Entrez Gene ID: 5788
  • Molecular mass of antigen [kDa]: 145
  • Cross-reactivity:
    chimpanzee (
    Pan troglodytes
    common marmoset (
    Callithrix jacchus
  • Distribution of antigen: thymocytes, granulocytes, T cells, B cells, monocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, APC-Vio770
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