CD45RO antibodies, human

CD45RO antibodies, human

Clone: REA611 | Dilution: 1:11
Clone REA611 recognizes the human CD45RO antigen, a 180 kDa single-chain membrane glycoprotein, which is an isoform of CD45. CD45RO is expressed by most thymocytes, activated and memory T cells, granulocytes, B cell subsets, and monocytes. CD45RO binds to galectin-1 and CD22. CD45RO antibodies are commonly used in combination with antibodies against CD45RA to discriminate memory T cells from naive T cells.
Additional information: Clone REA611 displays negligible binding to Fc receptors.

Technical specifications

  • Antigen: CD45RO
  • Clone: REA611
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Entrez Gene ID: 5788
  • Molecular mass of antigen [kDa]: 145
  • Cross-reactivity:
    chimpanzee (
    Pan troglodytes
    )
    ,
    common marmoset (
    Callithrix jacchus
    )
  • Distribution of antigen: thymocytes, granulocytes, T cells, B cells, monocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioGreen

Specificity

In order to compare the epitope specificity of REAfinity Clone REA611 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA611
UCHL1++
  • Selected references

    1. Charbonneau, H. et al. (1988) The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase. Proc. Natl. Acad. Sci. U.S.A. 85(19): 7182-7186
    2. Trowbridge, I. S. et al. (1994) CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. Annu. Rev. Immunol. 12: 85-116
Product options: 1
130-109-433

CD45RO-VioGreen, human

for 100
tests
(1)
-