CD44 antibodies, mouse

CD44 antibodies, mouse

Clone: REA664 | Dilution: 1:50
Clone REA664 recognizes the mouse CD44 antigen, which is also known as Pgp-1, HCELL, HUTCH-I, HCAM, HERMES, or Ly-24. CD44 is a 80–95 kDa membrane glycoprotein and expressed by a variety of hematopoetic and non-hematopoetic cells. Different isoforms are generated by alternative splicing. Clone REA664 reacts with all isoforms and both allelic forms of CD44. CD44 functions as a receptor for hyaluronic acid (HA) and mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). The interaction between galectin-9 and CD44 increases the stability and function of adaptive regulatory T cells. CD44 expression is upregulated on naive T cells upon activation and high CD44 expression is maintained on memory T cells. Therefore, CD44 is often used to discriminate CD44
high
antigen–experienced T cells from CD44
low
(CD62L
+
) naive T cells.
Additional information: Clone REA664 displays negligible binding to Fc receptors.

Alternative Names

CD44s, EMCR III, H-CA, Pgp-1, HERMES, Ly-24, HUTCH-I, Hermes antigen

Technical specifications

  • Antigen: CD44
  • Clone: REA664
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: CD44s, EMCR III, H-CA, Pgp-1, HERMES, Ly-24, HUTCH-I, Hermes antigen
  • Entrez Gene ID: 12505
  • Molecular mass of antigen [kDa]: 83
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
    ,
    chimpanzee (
    Pan troglodytes
    )
    , baboon, human, pig, cat, dog, cow, horse
  • Distribution of antigen: bone marrow, cancer cells, CNS cells, endothelial cells, epithelial cells, kidney, leukocytes, lymphocytes, mesenchymal stem cells, myeloid cells, plasma cells, ES and iPS cells, red blood cells, skeletal muscle, skin, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, PE-Vio770, APC-Vio770, VioBright FITC

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA664
IM7++
  • Selected References

    1. Ponta, H. et al. (2003) CD44: From adhesion molecules to signalling regulators. Nat. Rev. Mol. Cell Biol. 4(1): 33-45
    2. Wu, C. et al. (2014) Galectin-9-CD44 interaction enhances stability and function of adaptive regulatory T cells. Immunity 41(2): 270-282
    3. He, Q. et al. (1992) Molecular isoforms of murine CD44 and evidence that the membrane proximal domain is not critical for hyaluronate recognition. J. Cell Biol. 119(6): 1711-1719
Product options: 8

Product information

Size
Order no.
Price

CD44-PE, mouse

  • REA664
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-118-694
-

CD44-PE, mouse

  • REA664
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-118-566
-

CD44-APC, mouse

  • REA664
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-119-121
-

CD44-PE-Vio770, mouse

  • REA664
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-119-127
-

CD44-PE-Vio770, mouse

  • REA664
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-118-950
-

CD44-APC-Vio770, mouse

  • REA664
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-118-695
-

CD44-VioBright FITC, mouse

  • REA664
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-120-287
-

CD44-VioBright FITC, mouse

  • REA664
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-120-213
-