CD33 antibodies, human

CD33 antibodies, human

Clone: REA775 | Dilution: 1:50
Clone REA775 recognizes the human CD33 antigen, a 67 kDa glycoprotein belonging to the sialoadhesin superfamily. The CD33 antigen is highly expressed on human monocytes but weakly on granulocytes and some – but not all – myeloid dendritic cells. The CD33 antigen is also found on myeloid progenitor cells (CFU-GEMM, CFU GM, CFU-G, BFU-E) but is not expressed on lymphocytes, platelets, erythrocytes, or primitive hematopoietic stem cells.
Additional information: Clone REA775 displays negligible binding to Fc receptors.

Alternative names

Siglec-3, p67, My9

Technical specifications

  • Antigen: CD33
  • Clone: REA775
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: Siglec-3, p67, My9
  • Entrez Gene ID: 945
  • Molecular mass of antigen [kDa]: 38
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
    ,
    rhesus monkey (
    Macaca mulatta
    )
  • Distribution of antigen: basophils, dendritic cells, granulocytes, Langerhans cells, leukemia cells, macrophages, mast cells, monocytes, myeloid cells, NK cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, VioBright 515, Biotin
  • Selected references

    1. Simmons, D. et al. (1988) Isolation of a cDNA encoding CD33, a differentiation antigen of myeloid progenitor cells. J. Immunol. 141(8): 2797-2800
    2. Bernstein, I. D. et al. (1987)
      Treatment of acute myeloid leukemia cells
      in vitro
      with a monoclonal antibody recognizing a myeloid differentiation antigen allows normal progenitor cells to be expressed.
      J. Clin. Invest. 79(4): 1153-1159
    3. Hernández-Caselles, T. et al. (2006) A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing. J. Leukoc. Biol. 79(1): 46-58
Product options: 22
130-111-135

CD33-FITC, human

for 30
tests
(1)
-
130-111-018

CD33-FITC, human

for 100
tests
(1)
-
130-111-136

CD33-PE, human

for 30
tests
(1)
-
130-111-019

CD33-PE, human

for 100
tests
(1)
-
130-111-137

CD33-APC, human

for 30
tests
(1)
-
130-111-020

CD33-APC, human

for 100
tests
(1)
-
130-111-141

CD33-VioBlue, human

for 30
tests
(1)
-
130-111-024

CD33-VioBlue, human

for 100
tests
(1)
-
130-111-142

CD33-VioGreen, human

for 30
tests
(1)
-
130-111-025

CD33-VioGreen, human

for 100
tests
(1)
-
130-111-143

CD33-PE-Vio615, human

for 30
tests
(1)
-
130-111-026

CD33-PE-Vio615, human

for 100
tests
(1)
-
130-111-138

CD33-PE-Vio770, human

for 30
tests
(1)
-
130-111-021

CD33-PE-Vio770, human

for 100
tests
(1)
-
130-111-139

CD33-APC-Vio770, human

for 30
tests
(1)
-
130-111-022

CD33-APC-Vio770, human

for 100
tests
(1)
-
130-111-140

CD33-PerCP-Vio700, human

for 30
tests
(1)
-
130-111-023

CD33-PerCP-Vio700, human

for 100
tests
(1)
-
130-111-144

CD33-VioBright 515, human

for 30
tests
(1)
-
130-111-027

CD33-VioBright 515, human

for 100
tests
(1)
-
130-111-134

CD33-Biotin, human

for 30
tests
(1)
-
130-111-017

CD33-Biotin, human

for 100
tests
(1)
-