CD324 (E-Cadherin) antibodies, human

CD324 (E-Cadherin) antibodies, human

Clone: 67A4 | Dilution: 1:11
CD324, also known as E-Cadherin or cadherin-1, is a calcium-dependent cell adhesion protein found in adherence junctions of epithelial cells, e.g., in colon, uterus, liver, keratinocytes, etc. CD324 also serves as a ligand for integrin alpha-E/beta-7. The epitope is expressed by a variety of carcinoma-derived cell lines such as MCF-7, HT-29, T-47D, NTERA-2, and 2102Ep. Loss of CD324 is thought to enable metastasis by disrupting intercellular contacts – an early step in metastatic dissemination. Furthermore, CD324 is a marker of undifferentiated embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. For these cells, CD324 plays a major role in conveying cell survival signals.

Alternative Names

CDH1, Arc-1, CDHE, ECAD, LCAM, UVO, E-Cadherin

Technical specifications

  • Antigen: CD324 (E-Cadherin)
  • Clone: 67A4
  • Isotype: mouse IgG1κ
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Alternative names of antigen: CDH1, Arc-1, CDHE, ECAD, LCAM, UVO, E-Cadherin
  • Entrez Gene ID: 999
  • Molecular mass of antigen [kDa]: 80
  • Distribution of antigen: epithelial cells, platelets, ES and iPS cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, PE-Vio770, APC-Vio770
  • Selected References

    1. Higgins, J. M. et al. (1998) Direct and regulated interaction of integrin alphaEbeta7 with E-cadherin. J. Cell Biol. 140: 197-210
    2. Onder, T. T. et al. (2008) Loss of E-cadherin promotes metastasis via multiple downstream transcriptional pathways. Cancer Res. 68: 3645-3654
    3. Eastham, A. M. et al. (2007) Epithelial-mesenchymal transition events during human embryonic stem cell differentiation. Cancer Res. 67: 11254-11262
    4. Li, Z. et al. (2010) Spatially resolved quantification of E-cadherin on target hES cells. J. Phys. Chem. B 114: 2894-2900
    5. Xu, Y. et al. (2010) Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc. Natl. Acad. Sci. U.S.A. 107(18): 5129-8134
  • Brochures and posters

Product options: 8

Product information

Size
Order no.
Price

CD324 (E-Cadherin)-PE, human

  • 67A4
  • 1:11
  • For research use only
for 30
tests
(1)
130-099-688
-

CD324 (E-Cadherin)-PE, human

  • 67A4
  • 1:11
  • For research use only
for 100
tests
(1)
130-095-413
-

CD324 (E-Cadherin)-APC, human

  • 67A4
  • 1:11
  • For research use only
for 30
tests
(1)
130-099-723
-

CD324 (E-Cadherin)-APC, human

  • 67A4
  • 1:11
  • For research use only
for 100
tests
(1)
130-095-412
-

CD324 (E-Cadherin)-PE-Vio770, human

  • 67A4
  • 1:11
  • For research use only
for 30
tests
(1)
130-099-142
-

CD324 (E-Cadherin)-PE-Vio770, human

  • 67A4
  • 1:11
  • For research use only
for 100
tests
(1)
130-099-141
-

CD324 (E-Cadherin)-APC-Vio770, human

  • 67A4
  • 1:11
  • For research use only
for 30
tests
(1)
130-101-095
-

CD324 (E-Cadherin)-APC-Vio770, human

  • 67A4
  • 1:11
  • For research use only
for 100
tests
(1)
130-101-148
-