CD317 (PDCA-1) antibodies, mouse

CD317 (PDCA-1) antibodies, mouse

Clone: JF05-1C2.4.1 | Dilution: 1:50
Clone JF05-1C2.4.1 specifically recognizes the mouse CD317 antigen, also known as plasmacytoid dendritic cell antigen-1 (PDCA-1). CD317 is specifically expressed on mouse PDCs, a subset of CD11c
+
dendritic cells detected at low frequency in all lymphoid tissues, peripheral blood, and some non-lymphoid tissues.
In mouse spleen, bone marrow, and lymph nodes, CD317 is exclusively expressed on cells which are CD11c
low
, Ly-6C
high
, CD45R (B220)
+
, MHC class II
low
, CD8a
low/–
, CD80
low/–
, CD86
, CD40
, CD11b
, CD90
, CD49b (DX5)
, CD3
, CD19
, i.e. on cells with the phenotype of mouse PDCs. Multi-color fluorescent staining of spleen cells clearly revealed that all CD11c
low
Ly-6C
high
CD45R (B220)
+
PDCs are PDCA-1
+
and that PDCA-1 expression is restricted to PDCs.

Alternative Names

BST2, Bst-2, C87040, mPDCA-1, DAMP-1, GREG

Technical specifications

  • Antigen: CD317 (PDCA-1)
  • Clone: JF05-1C2.4.1
  • Isotype: rat IgG2b
  • Isotype control: Rat IgG2b – isotype control antibodies
  • Alternative names of antigen: BST2, Bst-2, C87040, mPDCA-1, DAMP-1, GREG
  • Entrez Gene ID: 69550
  • Molecular mass of antigen [kDa]: 17
  • Distribution of antigen: dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
  • Selected References

    1. Julian, M. W. et al. (2012) Mitochondrial transcription factor A serves as a danger signal by augmenting plasmacytoid dendritic cell responses to DNA. J. Immunol. 189(1): 433-443
    2. Matsui, H. et al. (2012) Mechanism of action of inhibition of allergic immune responses by a novel antedrug TLR7 agonist. J. Immunol. 189(11): 5194-5205
    3. Krug, A. et al. (2004) TLR9-dependent recognition of MCMV by IPC and DC generates coordinated cytokine responses that activate antiviral NK cell function. Immunity 21: 107-119
    4. Colonna, M. et al. (2004) Plasmacytoid dendritic cells in immunity. Nat. Immunol. 5: 1219-1226
    5. Yoo, J. K. et al. (2010) Identification of a novel antigen-presenting cell population modulating antiinfluenza type 2 immunity. J. Exp. Med. 207(7): 1435-1451
    6. Fallarino F et al. (2005) Ligand and cytokine dependence of the immunosuppressive pathway of tryptophan catabolism in plasmacytoid dendritic cells. Int. Immunol. 17(11): 1429-1438
    7. Xu, H. et al. (2007) Identification of novel dendritic cell populations in normal mouse retina. Invest. Ophthalmol. Vis. Sci. 48(4): 1701-1710
    8. Fischer, J. et al. (2004) mPDCA-1: A presumably novel antigen exclusively expressed by murine plasmacytoid dendritic cells. MACS&more 8(1): 13-15
    9. Daito, H. et al. (2011)
      Mycobacterial hypersensitivity pneumonitis requires TLR9-MyD88 in lung CD11b
      +
      CD11c
      +
      cells.
      Eur. Respir. J. 38(3): 688-701