CD314 (NKG2D) antibodies, human

CD314 (NKG2D) antibodies, human

Clone: BAT221 | Dilution: 1:11
Clone BAT221 recognizes CD314 (NKG2D), a cell surface receptor of the type II transmembrane receptor NKG2 family. It is expressed on natural killer (NK) cells, CD8
+
α/β T cells, and γ/δ T cells. Though NKG2D itself possesses no intrinsic signaling capability, ligand engagement while associated to the adaptor proteins DAP10 or DAP12 induces cytolytic activation in NK cells as well as costimulation in certain T cell populations. Ligands for NKG2D are stress-inducible MHC class I–related MICA, MICB, and UL16-binding proteins, expressed predominantly on epithelial cells and numerous carcinomas.

Alternative Names

Klrk1, KLR, NKG2D

Technical specifications

  • Antigen: CD314 (NKG2D)
  • Clone: BAT221
  • Isotype: mouse IgG1
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Alternative names of antigen: Klrk1, KLR, NKG2D
  • Entrez Gene ID: 22914
  • Molecular mass of antigen [kDa]: 25
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Distribution of antigen: macrophages, myeloid cells, NK cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, PE-Vio770, Biotin, pure
  • Selected References

    1. Warren, H. S. (2005) The Eighth Human Leucocyte Differentiation Antigen (HLDA8) Workshop: Natural killer cell section report. Cell. Immunol. 236: 17-20
    2. Moretta, L. and Moretta, A. (2004) Unravelling natural killer function: Triggering and inhibitory human NK receptors. EMBO J. 23: 255-259
    3. Groh, V. et al. (2003) Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis. Proc. Natl. Acad. Sci. U.S.A. 100(16): 9452-9457
    4. Bauer, S. et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science 285: 727-729
    5. Maasho, K. et al. (2005)
      NKG2D is a costimulatory receptor for human naive CD8
      +
      T cells.
      J. Immunol. 174: 4480-4484
    6. Chaput, N. et al. (2013)
      Phase I clinical trial combining imatinib mesylate and IL-2: HLA-DR
      +
      NK cell levels correlate with disease outcome.
      Oncoimmunology 2(2): e23080
Product options: 8
130-104-888

CD314 (NKG2D)-VioBright FITC, human

for 30
tests
(1)
-
130-104-842

CD314 (NKG2D)-VioBright FITC, human

for 100
tests
(1)
-
130-099-216

CD314 (NKG2D)-PE, human

for 30
tests
(1)
-
130-092-672

CD314 (NKG2D)-PE, human

for 100
tests
(1)
-
130-104-243

CD314 (NKG2D)-PE-Vio770, human

for 30
tests
(1)
-
130-104-209

CD314 (NKG2D)-PE-Vio770, human

for 100
tests
(1)
-
130-092-825

CD314 (NKG2D)-Biotin, human

for 100
tests
(1)
-
130-092-686

CD314 (NKG2D) pure, human

100 µg in 1 mL
-