CD303 (BDCA-2) antibodies, human

CD303 (BDCA-2) antibodies, human

Clone: REA693 | Dilution: 1:50
CD303 (BDCA-2) antibodies have been used, for example, to identify, characterize, and enumerate plasmacytoid dendritic cells in whole blood of healthy and HIV-infected individuals, and for analyzing the role of DC-SIGN in HIV infection and transmission. Furthermore, CD303 (BDCA-2) antibodies have been used to identify and enumerate plasmacytoid dendritic cells in blood and bone marrow samples before and after hematopoietic stem cell mobilization or transplantation. CD303 (BDCA-2) antibodies have also been used for immunohistochemical staining, for example, to identify plasmacytoid dendritic cells in tissue sections from patients with different inflammatory skin diseases.
Clone REA693 recognizes the CD303 (BDCA-2) antigen which is expressed on human plasmacytoid dendritic cells in blood, lymphoid (e.g. tonsils and bone marrow), and non-lymphoid tissue. Specific expression allows direct identification of plasmacytoid dendritic cells using just one marker. CD303 (BDCA-2)
+
plasmacytoid dendritic cells in blood and bone marrow are CD11c
, CD123
high
, CD4
+
, Lin
, CD45RA
+
, CD304 (BDCA-4/Neuropilin-1)
+
, CD141 (BDCA-3)
low
, CD1c (BDCA-1)
, CD14
, and CD2
. They express neither myeloid lineage markers (CD13, CD33) nor Fc receptors (CD32, CD64, FcRI). CD303 (BDCA-2) is strongly expressed on freshly isolated plasmacytoid dendritic cells but down-regulated within 48 hours of culturing. Unlike CD304 (BDCA-4/Neuropilin-1), CD303 (BDCA-2) is not detectable on
ex vivo
generated monocyte-derived or hematopoietic precursor cell-derived CD1a
+
dendritic cells.
The CD303 (BDCA-2) antigen is a novel type II transmembrane C-type lectin. Remarkably, plasmacytoid dendritic cells can take up ligands via CD303 (BDCA-2), then process and present the ligands to T cells. Unlike binding of antibodies to CD304 (BDCA-4), binding of antibodies to CD303 (BDCA-2) inhibits type I IFN production, which is induced in plasmacytoid dendritic cells by, for example, the influenza virus.
Additional information: Clone REA693 displays negligible binding to Fc receptors.

Alternative Names

BDCA-2

Technical specifications

  • Antigen: CD303 (BDCA-2)
  • Clone: REA693
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: BDCA-2
  • Entrez Gene ID: 170482
  • Molecular mass of antigen [kDa]: 25
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Distribution of antigen: dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, PE-Vio770, APC-Vio770, Biotin
Product options: 12

Product information

Size
Order no.
Price

CD303 (BDCA-2)-VioBright FITC, human

  • REA693
  • 1:50
  • For research use only
for 30
tests
(1)
130-114-182
-

CD303 (BDCA-2)-VioBright FITC, human

  • REA693
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-656
-

CD303 (BDCA-2)-PE, human

  • REA693
  • 1:50
  • For research use only
for 30
tests
(1)
130-114-180
-

CD303 (BDCA-2)-PE, human

  • REA693
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-654
-

CD303 (BDCA-2)-APC, human

  • REA693
  • 1:50
  • For research use only
for 30
tests
(1)
130-114-177
-

CD303 (BDCA-2)-APC, human

  • REA693
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-651
-

CD303 (BDCA-2)-PE-Vio770, human

  • REA693
  • 1:50
  • For research use only
for 30
tests
(1)
130-114-181
-

CD303 (BDCA-2)-PE-Vio770, human

  • REA693
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-655
-

CD303 (BDCA-2)-APC-Vio770, human

  • REA693
  • 1:50
  • For research use only
for 30
tests
(1)
130-114-178
-

CD303 (BDCA-2)-APC-Vio770, human

  • REA693
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-652
-

CD303 (BDCA-2)-Biotin, human

  • REA693
  • 1:50
  • For research use only
for 30
tests
(1)
130-114-179
-

CD303 (BDCA-2)-Biotin, human

  • REA693
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-653
-

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