CD303 (BDCA-2) antibodies, human

CD303 (BDCA-2) antibodies, human

Clone: AC144
CD303 (BDCA-2) antibodies have been used, for example, to identify, characterize, and enumerate plasmacytoid dendritic cells in whole blood of healthy and HIV-infected individuals, and for analyzing the role of DC-SIGN in HIV infection and transmission.
7,11
Furthermore, CD303 (BDCA-2) antibodies were used to identify and enumerate plasmacytoid dendritic cells in blood and bone marrow samples before and after hematopoietic stem cell mobilization
8
or transplantation
10
. CD303 (BDCA-2) antibodies were also used for immunohistochemical staining, for example to identify plasmacytoid dendritic cells in tissue sections from patients with different inflammatory skin diseases.
5,12,13
Clone AC144 recognizes the the CD303 (BDCA-2) antigen
1,3
which is expressed on human plasmacytoid dendritic cells in blood, lymphoid (e.g. tonsils and bone marrow) and non-lymphoid tissue.
3,6
Specific expression allows direct identification of plasmacytoid dendritic cells using just one marker.
1–11
CD303 (BDCA-2)
+
plasmacytoid dendritic cells in blood and bone marrow are CD11c
, CD123
high
, CD4
+
, Lin
, CD45RA
+
, CD304 (BDCA-4/Neuropilin-1)
+
, CD141 (BDCA-3)
low
, CD1c (BDCA-1)
, CD14
, and CD2
. They express neither myeloid lineage markers (CD13, CD33) nor Fc receptors (CD32, CD64, FcRI).
1,6
CD303 (BDCA-2) is strongly expressed on freshly isolated plasmacytoid dendritic cells but down-regulated within 48 hours of culturing.
1
Unlike CD304 (BDCA-4/Neuropilin-1), CD303 (BDCA-2) is not detectable on
ex vivo
generated monocyte-derived or hematopoietic precursor cell-derived CD1a
+
dendritic cells.
1,9
The CD303 (BDCA-2) antigen is a novel type II transmembrane C-type lectin.
3
Remarkably, plasmacytoid dendritic cells can take up ligands via CD303 (BDCA-2), then process and present the ligands to T cells.
1,3
Unlike binding of antibodies to CD304 (BDCA-4), binding of antibodies to CD303 (BDCA-2) inhibits type I IFN production, which is induced in plasmacytoid dendritic cells by, for example, the influenza virus.
3,6

Alternative Names

CLEC4C, BDCA-2, CLECSF11, CLECSF7, DLEC, HECL, PRO34150

Technical specifications

  • Antigen: CD303 (BDCA-2)
  • Clone: AC144
  • Isotype: mouse IgG1
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Alternative names of antigen: CLEC4C, BDCA-2, CLECSF11, CLECSF7, DLEC, HECL, PRO34150
  • Entrez Gene ID: 170482
  • Molecular mass of antigen [kDa]: 25
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Distribution of antigen: dendritic cells
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
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