CD3 antibodies, mouse

CD3 antibodies, mouse

Clone: REA641 | Dilution: 1:50
Clone REA641 recognizes the mouse CD3 complex, which is expressed on all mature T lymphocytes, NKT cells, and during the development of thymocytes. CD3, also known as T3, belongs to the Ig superfamily. It consists of CD3ε, δ, γ, and ζ chains and forms a TCR complex by associating with TCR α/β or γ/δ chains. The TCR does not possess intracellular signaling domains, uncoupling Ag recognition from T cell signaling. The TCR is instead non-covalently associated with a multisubunit signaling apparatus, consisting of the CD3εγ and CD3εδ heterodimers and the CD3ζζ homodimer, which collectively form the TCR-CD3 complex. CD3 is involved in T cell activation, signal transduction, and antigen recognition by binding the peptide/MHC antigen complex.
Additional information: Clone REA641 displays negligible binding to Fc receptors.

Alternative Names

T cell antigen receptor complex, T3, AI504783, CD3epsilon, T3E, Ctg-3, Ctg3, T3g

Technical specifications

  • Antigen: CD3
  • Clone: REA641
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: T cell antigen receptor complex, T3, AI504783, CD3epsilon, T3E, Ctg-3, Ctg3, T3g
  • Entrez Gene ID: 12501, 12502
  • Molecular mass of antigen [kDa]: 37
  • Distribution of antigen: NKT cells, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE-Vio770, FITC, APC-Vio770
REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 
Other clonesOverlap in epitope recognition with REA641
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