CD3 antibodies, human

CD3 antibodies, human

Clone: REA613 | Dilution: 1:50
Clone REA613 recognizes the human CD3 antigen, a single-pass type I membrane protein, also known as Leu4. CD3 is present on mature human T cells, thymocytes, a subset of natural killer (NK) cells, and on Purkinje cells in cerebellum. It is associated with the T cell receptor (TCR) and is responsible for its signal transduction. The CD3 antigen is a complex of five invariable chains: γ, δ, ε, ζ, and η. The epitope recognized by the antibody is located on the ε-chain of the CD3 complex.
Additional information: Clone REA613 displays negligible binding to Fc receptors.

Alternative names

CD3-epsilon, CD3e, Leu4, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, IMD18, TCRE

Technical specifications

  • Antigen: CD3
  • Clone: REA613
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: CD3-epsilon, CD3e, Leu4, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, IMD18, TCRE
  • Entrez Gene ID: 916
  • Molecular mass of antigen [kDa]: 21
  • Distribution of antigen: leukocytes, NK cells, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA613 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA613
BW264/56++
UCHT1++
HIT3++
SK7++
OKT3++
SP34-2+
  • Selected references

    1. Gold, D. P. et al. (1986) Isolation of cDNA clones encoding the 20K non-glycosylated polypeptide chain of the human T-cell receptor/T3 complex. Nature 321(6068): 431-434
    2. Birnbaum, M. E. et al. (2014) Molecular architecture of the αβ T cell receptor-CD3 complex. Proc. Natl. Acad. Sci. U.S.A. 111(49): 17576-17581
    3. D'Acquisto, F. et al. (2011)
      CD3
      +
      CD4
      -
      CD8
      -
      (double negative) T cells: saviours or villains of the immune response?
      Biochem. Pharmacol. 82(4): 333-340
Product options: 20
130-113-700

CD3-FITC, human

for 30
tests
(1)
-
130-113-138

CD3-FITC, human

for 100
tests
(1)
-
130-113-701

CD3-PE, human

for 30
tests
(1)
-
130-113-139

CD3-PE, human

for 100
tests
(1)
-
130-113-697

CD3-APC, human

for 30
tests
(1)
-
130-113-135

CD3-APC, human

for 100
tests
(1)
-
130-114-710

CD3-VioBlue, human

for 30
tests
(1)
-
130-114-519

CD3-VioBlue, human

for 100
tests
(1)
-
130-113-704

CD3-VioGreen, human

for 30
tests
(1)
-
130-113-142

CD3-VioGreen, human

for 100
tests
(1)
-
130-114-711

CD3-PE-Vio615, human

for 30
tests
(1)
-
130-114-520

CD3-PE-Vio615, human

for 100
tests
(1)
-
130-113-702

CD3-PE-Vio770, human

for 30
tests
(1)
-
130-113-140

CD3-PE-Vio770, human

for 100
tests
(1)
-
130-113-698

CD3-APC-Vio770, human

for 30
tests
(1)
-
130-113-136

CD3-APC-Vio770, human

for 100
tests
(1)
-
130-113-703

CD3-PerCP-Vio700, human

for 30
tests
(1)
-
130-113-141

CD3-PerCP-Vio700, human

for 100
tests
(1)
-
130-113-699

CD3-Biotin, human

for 30
tests
(1)
-
130-113-137

CD3-Biotin, human

for 100
tests
(1)
-