CD28 antibodies – functional grade, human

CD28 antibodies – functional grade, human

Clone: 15E8
The CD28 antibody recognizes the human CD28 antigen, which is highly expressed on CD3
+
thymocytes, most peripheral thymocytes, and plasma cells. Binding of CD28 to its ligands CD80 or CD86 costimulates T cell effector function and T cell–dependent antibody production
in vitro
and
in vivo
.
In the presence of antibodies directed against CD2 and CD3, CD28 antibodies stimulate T cell proliferation and cytokine production.
Activation and proliferation of T cells can be induced by interaction of the T cell receptor with peptide-MHC complexes expressed on the surface of antigen-presenting cells (APCs). The T cell receives a signal transduced through the CD3 complex. Cytokines or other costimulatory signals are required in addition. Activated T cells can be used for any downstream processes, such as cytokine analysis or immunoprecipitation. Cells can also be transfected with high efficiency.

Applications

The CD28 antibodies – functional grade are suited for functional assays, for example,
in vitro
activation and expansion of T cells in combination with CD2 and CD3 pure – functional grade antibodies, or CD2- and CD3-Biotin – functional grade antibodies, and Anti-Biotin MACSiBead™ Particles, cell culture grade.

Alternative Names

Tp44, T44

Technical specifications

  • Antigen: CD28
  • Clone: 15E8
  • Isotype: mouse IgG1
  • Alternative names of antigen: Tp44, T44
  • Entrez Gene ID: 940
  • Molecular mass of antigen [kDa]: 23
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Distribution of antigen: B cells, lymphocytes, plasma cells, T cells, thymocytes
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: Biotin, pure
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