CD278 (ICOS) antibodies, human, mouse, rat

CD278 (ICOS) antibodies, human, mouse, rat

Clone: REA192 | Dilution: 1:11
Clone REA192 recognizes CD278 (ICOS), which belongs to the CD28 family of proteins. CD28 family members are co-stimulatory receptor molecules, contain single immunoglobulin V-like domain, and belong to the immunoglobulin superfamily. CD287 recognizes ICOSL (inducible T cell co-stimulator ligand) as ligand and its expression is induced on activated T cells. Enhanced expression of CD278 is also observed on T cells, followed by co-stimulation of T cells via CD28. Further expression of CD278 is found on B cells, monocytes, and dendritic cells. Crosslinking of ICOS on T cell surface in the presence of primary stimulation signal leads to T cell proliferation and differentiation. In addition, CD278 plays role in Tʜ cell differentiation, effector T cell function, and isotype class switching.
Additional information: Clone REA192 displays negligible binding to Fc receptors.

Alternative Names

ICOS, AILIM, CVID1

Technical specifications

  • Antigen: CD278 (ICOS)
  • Clone: REA192
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: ICOS, AILIM, CVID1
  • Distribution of antigen: T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA192 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA192
DX29++
ISA-3++
C398.4A++
  • Selected References

    1. Akbari, O. et al. (2008)
      ICOS/ICOSL interaction is required for CD4
      +
      invariant NKT cell function and homeostatic survival
      J. Immunol. 180: 5448-5456
    2. Wassink, L. et al. (2004) ICOS expression by activated human Tʜ Cells is enhanced by IL-12 and IL-23: Increased ICOS expression enhances the effector function of both Tʜ1 and Tʜ2 cells. J. Immunol. 173: 1779-1786
Product options: 18
130-100-734

CD278 (ICOS)-FITC, human, mouse, rat

for 30
tests
(1)
-
130-100-732

CD278 (ICOS)-FITC, human, mouse, rat

for 100
tests
(1)
-
130-100-719

CD278 (ICOS)-APC, human, mouse, rat

for 30
tests
(1)
-
130-100-721

CD278 (ICOS)-APC, human, mouse, rat

for 100
tests
(1)
-
130-100-639

CD278 (ICOS)-VioBlue, human, mouse, rat

for 30
tests
(1)
-
130-100-737

CD278 (ICOS)-VioBlue, human, mouse, rat

for 100
tests
(1)
-
130-100-740

CD278 (ICOS)-VioGreen, human, mouse, rat

for 30
tests
(1)
-
130-100-739

CD278 (ICOS)-VioGreen, human, mouse, rat

for 100
tests
(1)
-
130-107-512

CD278 (ICOS)-PE-Vio615, human, mouse, rat

for 30
tests
(1)
-
130-107-458

CD278 (ICOS)-PE-Vio615, human, mouse, rat

for 100
tests
(1)
-
130-100-736

CD278 (ICOS)-PE-Vio770, human, mouse, rat

for 30
tests
(1)
-
130-100-735

CD278 (ICOS)-PE-Vio770, human, mouse, rat

for 100
tests
(1)
-
130-100-727

CD278 (ICOS)-APC-Vio770, human, mouse, rat

for 30
tests
(1)
-
130-100-723

CD278 (ICOS)-APC-Vio770, human, mouse, rat

for 100
tests
(1)
-
130-100-733

CD278 (ICOS)-PerCP-Vio700, human, mouse, rat

for 30
tests
(1)
-
130-100-731

CD278 (ICOS)-PerCP-Vio700, human, mouse, rat

for 100
tests
(1)
-
130-100-726

CD278 (ICOS)-Biotin, human, mouse, rat

for 30
tests
(1)
-
130-100-728

CD278 (ICOS)-Biotin, human, mouse, rat

for 100
tests
(1)
-