CD25 antibodies, mouse

CD25 antibodies, mouse

Clone: REA568 | Dilution: 1:10
Clone REA568 recognizes the mouse CD25 antigen, the α chain of the IL-2 receptor (IL-2R). IL-2Rα associates with the β chain (CD122) and the γ chain (CD132) to form the functional IL-2R complex. IL-2Rα is expressed on CD4
+
CD25
+
regulatory T cells, activated T and B cells, and to a lesser extent on activated dendritic cells. It is also transiently expressed during T and B cell development. Binding of the CD25 antibody does not impede the binding of IL-2 to its receptor. In combination with CD4, CD25 antibody can be used to identify regulatory T cells (Tregs).
Additional information: Clone REA568 displays negligible binding to Fc receptors.

Alternative Names

IL2RA, IL2R, Ly-43, p55

Technical specifications

  • Antigen: CD25
  • Clone: REA568
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: IL2RA, IL2R, Ly-43, p55
  • Entrez Gene ID: 16184
  • Molecular mass of antigen [kDa]: 28
  • Distribution of antigen: T cells, B cells, dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, PE-Vio770, Biotin
REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 
Other clonesOverlap in epitope recognition with REA568
3C7+
7D4++
PC61-
Product options: 10

Product information

Size
Order no.
Price

CD25-VioBright FITC, mouse

  • REA568
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-109-054
-

CD25-VioBright FITC, mouse

  • REA568
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-108-999
-

CD25-PE, mouse

  • REA568
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-109-051
-

CD25-PE, mouse

  • REA568
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-108-996
-

CD25-APC, mouse

  • REA568
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-109-052
-

CD25-APC, mouse

  • REA568
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-108-997
-

CD25-PE-Vio770, mouse

  • REA568
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-109-053
-

CD25-PE-Vio770, mouse

  • REA568
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-108-998
-

CD25-Biotin, mouse

  • REA568
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-109-050
-

CD25-Biotin, mouse

  • REA568
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-108-995
-

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