CD25 antibodies, human

CD25 antibodies, human

Clone: REA945 | Dilution: 1:50
Clone REA945 recognizes the human CD25 antigen, a 28 kDa glycoprotein also known as the low-affinity interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed on activated T and B cells, on macrophages, and on a subset of non-activated CD4
+
regulatory T cells. CD25 plays an essential role in the regulation of immune tolerance by controlling regulatory T cells activity. The CD25 antigen contains three epitope regions called A, B, and C. Clone REA945 recognizes epitope region B.
Additional information: Clone REA945 displays negligible binding to Fc receptors.

Alternative Names

IL2RA, IDDM10, IL2R, TCGFR, p55, Tac, IL-2Rα

Technical specifications

  • Antigen: CD25
  • Clone: REA945
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: IL2RA, IDDM10, IL2R, TCGFR, p55, Tac, IL-2Rα
  • Entrez Gene ID: 3559
  • Molecular mass of antigen [kDa]: 28
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
    ,
    rhesus monkey (
    Macaca mulatta
    )
  • Distribution of antigen: myeloid cells, B cells, granulocytes, lymphocytes, macrophages, monocytes, NK cells, osteoclasts, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio615, PE-Vio770, Biotin
REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 
Other clonesOverlap in epitope recognition with REA945
4E3++
3G10-
REA570-
2A3-
BC96-
M-A251++

Related products

3 products available
Product options: 9

Product information

Size
Order no.
Price

CD25-PE, human

  • REA945
  • 1:50
  • For research use only
for 30
tests
(1)
130-115-628
-

CD25-PE, human

  • REA945
  • 1:50
  • For research use only
for 100
tests
(1)
130-115-534
-

CD25-APC, human

  • REA945
  • 1:50
  • For research use only
for 100
tests
(1)
130-115-535
-

CD25-PE-Vio615, human

  • REA945
  • 1:50
  • For research use only
for 30
tests
(1)
130-115-631
-

CD25-PE-Vio615, human

  • REA945
  • 1:50
  • For research use only
for 100
tests
(1)
130-115-537
-

CD25-PE-Vio770, human

  • REA945
  • 1:50
  • For research use only
for 30
tests
(1)
130-116-308
-

CD25-PE-Vio770, human

  • REA945
  • 1:50
  • For research use only
for 100
tests
(1)
130-116-205
-

CD25-Biotin, human

  • REA945
  • 1:50
  • For research use only
for 30
tests
(1)
130-115-626
-

CD25-Biotin, human

  • REA945
  • 1:50
  • For research use only
for 100
tests
(1)
130-115-532
-