CD244 (2B4) antibodies, human

CD244 (2B4) antibodies, human

Clone: REA112 | Dilution: 1:11
Clone REA112 recognizes CD244, a type I transmembrane receptor belonging to the CD2 subfamily of the Ig superfamily. Expression of CD244 is found on all natural killer (NK) cells, large subset of CD8
+
T cells, a small fraction of CD4
+
T cells, and eosinophils. Human CD244 upon ligand engagement activates NK cell cytotoxicity and production of interferon gamma (IFN-γ). Activating function of human CD244 requires recruitment of SH2 domain containing molecule called SLAM associated protein (SAP). In humans, CD244 has also been implicated as an inhibitory receptor such as in developing NK cells. In contrast, murine CD244 is primarily involved in inhibiting NK cell functions. Two isoforms of human CD244 exist on NK cells, h2B4-A and h2B4-B, which differ in their binding affinity to ligand CD48.
Additional information: Clone REA112 displays negligible binding to Fc receptors.

Alternative Names

2B4, NAIL, NKR2B4, Nmrk, SLAMF4

Technical specifications

  • Antigen: CD244 (2B4)
  • Clone: REA112
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: 2B4, NAIL, NKR2B4, Nmrk, SLAMF4
  • Entrez Gene ID: 51744
  • Molecular mass of antigen [kDa]: 39
  • Distribution of antigen: NK cells, T cells, eosinophils
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, Biotin
  • Selected References

    1. Munitz, A. et al. (2005) 2B4 (CD244) is expressed and functional on human eosinophils. J. Immunol. 174: 110-118
    2. Sivori, S. et al. (2002) Early expression of triggering receptors and regulatory role of 2B4 in human natural killer cell precursors undergoing in vitro differentiation. Proc. Natl. Acad. Sci. U.S.A. 99: 4526-4531
    3. Mathew, S. O. et al. (2009) Functional role of human NK cell receptor 2B4 (CD244) isoforms. Eur. J. Immunol. 39(6): 1632-1641
    4. Vaidya, S. V. et al. (2006) Of mice and men: different functions of the murine and human 2B4 (CD244) receptor on NK cells. Immunol. Lett. 105(2): 180-184
Product options: 10
130-099-041

CD244 (2B4)-FITC, human

for 30
tests
(1)
-
130-099-039

CD244 (2B4)-FITC, human

for 100
tests
(1)
-
130-099-071

CD244 (2B4)-PE, human

for 30
tests
(1)
-
130-099-051

CD244 (2B4)-PE, human

for 100
tests
(1)
-
130-099-073

CD244 (2B4)-APC, human

for 30
tests
(1)
-
130-099-072

CD244 (2B4)-APC, human

for 100
tests
(1)
-
130-099-075

CD244 (2B4)-PE-Vio770, human

for 30
tests
(1)
-
130-099-074

CD244 (2B4)-PE-Vio770, human

for 100
tests
(1)
-
130-099-038

CD244 (2B4)-Biotin, human

for 30
tests
(1)
-
130-099-037

CD244 (2B4)-Biotin, human

for 100
tests
(1)
-