CD223 antibodies, human

CD223 antibodies, human

Clone: REA351 | Dilution: 1:50
Clone REA351 recognizes the human CD223 antigen, a single-pass type I membrane protein also known as lymphocyte activation gene 3 protein (LAG-3). CD223 is a CD4 homolog with four extracellular Ig superfamily domains. Similar to CD4, CD223 oligomerizes at the surfaces of T cells and binds to MHC II on antigen presenting cells but with significantly higher affinity than CD4. CD223 is expressed on activated CD4
+
and CD8
+
T lymphocytes where it associates with the CD3–TCR complex at the cell surface and negatively regulates signal transduction. As a consequence, it negatively regulates T cell proliferation, function, and homeostasis. There is also increasing evidence of its involvement in regulatory function of tumor-infiltrated T cells in cancer, such as in Hodgkin’s lymphomas and in prostate cancer. CD223 is also expressed on activated NK cells, B cells, and plasmacytoid dendritic cells.
Additional information: Clone REA351 displays negligible binding to Fc receptors.

Alternative Names

LAG-3, Protein FDC

Technical specifications

  • Antigen: CD223
  • Clone: REA351
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: LAG-3, Protein FDC
  • Entrez Gene ID: 3902
  • Molecular mass of antigen [kDa]: 54
  • Distribution of antigen: T cells, lymphocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBlue, Biotin, APC, PE

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA351
T47-530+
3DS223H++
11C3C65-
7H2C65+
17B4+
  • Selected References

    1. Huang, C. T. et al. (2004) Role of LAG-3 in regulatory T cells. Immunity 21(4): 503-513
    2. Triebel, F. et al. (1990) LAG-3, a novel lymphocyte activation gene closely related to CD4. J. Exp. Med. 171(5): 1393-1405
    3. Gagliani, N. et al. (2013) Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells. Nat. Med. 19(6): 739-746
Product options: 8
130-118-677

CD223-VioBlue, human

for 30
tests
(1)
-
130-118-549

CD223-VioBlue, human

for 100
tests
(1)
-
130-119-163

CD223-Biotin, human

for 30
tests
(1)
-
130-118-986

CD223-Biotin, human

for 100
tests
(1)
-
130-119-666

CD223-APC, human

for 30
tests
(1)
-
130-119-567

CD223-APC, human

for 100
tests
(1)
-
130-120-610

CD223-PE, human

for 30
tests
(1)
-
130-120-470

CD223-PE, human

for 100
tests
(1)
-