CD203c antibodies, human

CD203c antibodies, human

Clone: FR3-16A11 | Dilution: 1:50
Clone FR3-16A11 recognizes CD203c, a glycosylated type II transmembrane molecule that belongs to the family of ecto-nucleotide pyrophosphatase/ phosphodiesterase (E-NPP3) enzymes. Among hematopoietic cells, expression of CD203c is restricted to basophils, mast cells and their precursors, and has been described as specific for this lineage. Protein and/or mRNA expression of CD203c has also been found in solid tissues such as uterus or prostate. Basophils and mast cells are key producers of mediators that drive the onset of inflammatory responses, e.g. in allergy. Allergen challenge leads to a rapid up-regulation of activation markers such as CD203c or CD63. Due to its restricted expression pattern, CD203c is discussed as a specific marker to monitor the allergen-induced activation of basophils, e.g. in flow cytometric basophil activation tests of the peripheral blood.

Alternative Names

ENPP3, B10, NPP3, PD-IBETA, PDNP3, E-NPP3, PD-1b

Technical specifications

  • Antigen: CD203c
  • Clone: FR3-16A11
  • Isotype: mouse IgG1κ
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Alternative names of antigen: ENPP3, B10, NPP3, PD-IBETA, PDNP3, E-NPP3, PD-1b
  • Entrez Gene ID: 5169
  • Molecular mass of antigen [kDa]: 100
  • Distribution of antigen: basophils, liver, mast cells, myeloid cells, pancreas, small intestine
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
  • Selected References

    1. Bühring, H. J. et al. (1999) The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors. Blood 94: 2343-2356
    2. Goding, J. W. (2000) Ecto-enzymes: physiology meets pathology. J. Leukoc. Biol. 67: 285-311
    3. Kahlert, H. et al. (2003) Measurement of basophil-activating capacity of grass pollen allergens, allergoids Clin. Exp. Allergy 33: 1266-1272
    4. Bühring, H. J. et al. (2004) The basophil-specific ectoenzyme E-NPP3 (CD203c) as a marker for cell activation and allergy diagnosis. Int. Arch. Allergy Immunol. 133: 317-329
    5. Hauswirth, A. W. et al. (2002) Recombinant allergens promote expression of CD203c on basophils in sensitized individuals. J. Allergy Clin. Immunol. 110: 102-109