CD20 antibodies, human

CD20 antibodies, human

Clone: REA780 | Dilution: 1:50
Clone REA780 recognizes the CD20 antigen, a non-glycosylated transmembrane protein of 33–37 kDa that is expressed on B lineage cells from the pre–B cell stage to the B cell lymphoblast stage. The antigen is further expressed on most malignant B cells. CD20 is not found on early B cell progenitors or plasma cells. Oligomers of CD20 form a Ca
2+
channel and might function in the regulation of local responses during B cell activation.
Additional information: Clone REA780 displays negligible binding to Fc receptors.

Alternative Names

MS4A1, B1, Bp35, CVID5, LEU-16, MS4A2, S7, Ly-44

Technical specifications

  • Antigen: CD20
  • Clone: REA780
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: MS4A1, B1, Bp35, CVID5, LEU-16, MS4A2, S7, Ly-44
  • Entrez Gene ID: 931
  • Molecular mass of antigen [kDa]: 33
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
    ,
    rhesus monkey (
    Macaca mulatta
    )
  • Distribution of antigen: B cells, cancer stem cells, dendritic cells, lymphocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, VioBright 515, Biotin

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA780
LT20++
2H7++
Rituximab/Hu2++
LT27++
  • Selected References

    1. Countouriotis, T. B. et al. (2002) Cell surface antigen and molecular targeting in the treatment of hematologic malignancies. Stem Cells 20(3): 215-229
    2. Polyak et al. (2002) CD20 Workshop Panel report. In: Leucocyte typing VII, Oxford University Press.
    3. Lamprecht, B. et al. (2008) Aberrant expression of the Tʜ2 cytokine IL-21 in Hodgkin lymphoma cells regulates STAT3 signaling and attracts Treg cells via regulation of MIP-3alpha. Blood 112(8): 3339-3347
    4. Maxwell, S. A. et al. (2009) 14-3-3zeta J. Biol. Chem. 284(33): 22379-22389
Product options: 22

Product information

Size
Order no.
Price

CD20-FITC, human

  • REA780
  • 1:50
  • For research use only
for 30
tests
(1)
130-111-523
-

CD20-FITC, human

  • REA780
  • 1:50
  • For research use only
for 100
tests
(1)
130-111-337
-

CD20-PE, human

  • REA780
  • 1:50
  • For research use only
for 30
tests
(1)
130-111-524
-

CD20-PE, human

  • REA780
  • 1:50
  • For research use only
for 100
tests
(1)
130-111-338
-

CD20-APC, human

  • REA780
  • 1:50
  • For research use only
for 30
tests
(1)
130-111-525
-

CD20-APC, human

  • REA780
  • 1:50
  • For research use only
for 100
tests
(1)
130-111-339
-

CD20-VioBlue, human

  • REA780
  • 1:50
  • For research use only
for 30
tests
(1)
130-111-531
-

CD20-VioBlue, human

  • REA780
  • 1:50
  • For research use only
for 100
tests
(1)
130-111-345
-

CD20-VioGreen, human

  • REA780
  • 1:50
  • For research use only
for 30
tests
(1)
130-111-532
-

CD20-VioGreen, human

  • REA780
  • 1:50
  • For research use only
for 100
tests
(1)
130-111-346
-

CD20-PE-Vio615, human

  • REA780
  • 1:50
  • For research use only
for 30
tests
(1)
130-111-533
-

CD20-PE-Vio615, human

  • REA780
  • 1:50
  • For research use only
for 100
tests
(1)
130-111-347
-

CD20-PE-Vio770, human

  • REA780
  • 1:50
  • For research use only
for 30
tests
(1)
130-111-526
-

CD20-PE-Vio770, human

  • REA780
  • 1:50
  • For research use only
for 100
tests
(1)
130-111-340
-

CD20-APC-Vio770, human

  • REA780
  • 1:50
  • For research use only
for 30
tests
(1)
130-111-527
-

CD20-APC-Vio770, human

  • REA780
  • 1:50
  • For research use only
for 100
tests
(1)
130-111-341
-

CD20-PerCP-Vio700, human

  • REA780
  • 1:50
  • For research use only
for 30
tests
(1)
130-111-528
-

CD20-PerCP-Vio700, human

  • REA780
  • 1:50
  • For research use only
for 100
tests
(1)
130-111-342
-

CD20-VioBright 515, human

  • REA780
  • 1:50
  • For research use only
for 30
tests
(1)
130-111-529
-

CD20-VioBright 515, human

  • REA780
  • 1:50
  • For research use only
for 100
tests
(1)
130-111-343
-

CD20-Biotin, human

  • REA780
  • 1:50
  • For research use only
for 30
tests
(1)
130-111-522
-

CD20-Biotin, human

  • REA780
  • 1:50
  • For research use only
for 100
tests
(1)
130-111-336
-