CD1c (BDCA-1) antibodies, human

CD1c (BDCA-1) antibodies, human

Clone: REA694 | Dilution: 1:50
Clone REA694 recognizes the human CD1c antigen, also known as BDCA-1. CD1c is expressed on a major subpopulation of human myeloid dendritic cells (about 0.3% of blood leukocytes). Blood CD1c (BDCA-1)
+
myeloid dendritic cells are CD11c
high
, CD123
low
, CD4
+
, Lin
, CD45RO
+
, CD2
+
, CD16
, CD141 (BDCA-3)
low
, CD303 (BDCA-2)
, and CD304 (BDCA-4/Neuropilin-1)
. They express myeloid markers (CD13, CD33) as well as Fc receptors (CD32, CD64, FcεRI) and are of monocytoid appearance. A minor proportion of CD1c (BDCA-1)
+
myeloid dendritic cells expresses CD14 and CD11b. In blood, CD1c (BDCA-1) is also expressed on a subpopulation of CD19
+
small resting B lymphocytes. CD1c (BDCA-1) expression has also been detected on cortical thymocytes, on Langerhans cells, and on CD1a
+
dendritic cells generated
ex vivo
from monocytes or hematopoietic precursor cells. CD1c (BDCA-1)
+
myeloid dendritic cells have been designated as type-1 myeloid dendritic cells (MDC1s). The CD1c antigen is a member of the CD1 family of proteins that are structurally related to MHC class I proteins and mediate the presentation of non-peptide antigens to T cells.
Additional information: Clone REA694 displays negligible binding to Fc receptors.

Alternative Names

BDCA-1, CD1, R7, M241, T6, CD1a

Technical specifications

  • Antigen: CD1c (BDCA-1)
  • Clone: REA694
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: BDCA-1, CD1, R7, M241, T6, CD1a
  • Entrez Gene ID: 911
  • Molecular mass of antigen [kDa]: 36
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Distribution of antigen: dendritic cells, B cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, PE-Vio770, Biotin

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA694
AD5-8E7++
L161-
F10/21A3-
  • Selected References

    1. Carrion, J. et al. (2012) Microbial carriage state of peripheral blood dendritic cells (DCs) in chronic periodontitis influences DC differentiation, atherogenic potential. J. Immunol. 189(6): 3178-3187
    2. Clark, F. J. et al. (2003) Origin and subset distribution of peripheral blood dendritic cells in patients with chronic graft-versus-host disease. Transplantation 75: 221-225
    3. Krupna-Gaylord, M. A. et al. (2014) Induction of type I and type III interferons by Borrelia burgdorferi correlates with pathogenesis and requires linear plasmid 36. PLoS One 9(6): e100174
    4. Peiser, M. et al. (2003) CD1a and CD1c cell sorting yields a homogeneous population of immature human Langerhans cells. J. Immunol. Methods 279: 41-53
    5. Grabbe, S. et al. (2000) Dendritic cells: multi-lineal and multi-functional. Immunol. Today 21: 431-433
    6. Brigl, M. and Brenner, M. B. (2004) CD1: antigen presentation and T cell function. Annu. Rev. Immunol. 22: 817-890
    7. Hémont, C. et al. (2013) Human blood mDC subsets exhibit distinct TLR repertoire and responsiveness. J. Leukoc. Biol. 93(4): 599-609
    8. Brodie Miles, E. et al. (2013) Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen. J. Leukoc. Biol. 94(2): 281-289
    9. MacDonald, K. P. A. et al. (2002) Characterization of human blood dendritic cell subsets. Blood 100: 4512-4520
    10. van de Ven, R. et al. (2011) Characterization of four conventional dendritic cell subsets in human skin-draining lymph nodes in relation to T-cell activation. Blood 118(9): 2502-2510
    11. Hou, W. et al. (2012) Viral infection triggers rapid differentiation of human blood monocytes into dendritic cells. Blood 119(13): 3128-3131
    12. Bosco, M. C. et al. (2011)
      Hypoxia modulates the gene expression profile of immunoregulatory receptors in human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker
      in vitro
      and
      in vivo
      .
      Blood 117(9): 2625-2639
    13. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
    14. Mittag, D. et al. (2011) Human dendritic cell subsets from spleen and blood are similar in phenotype and function but modified by donor health status. J. Immunol. 186(11): 6207-6217
    15. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
    16. Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species. J. Immunol. 185(6): 3313-3325
    17. Vanders, R. L. et al. (2013) Plasmacytoid dendritic cells and CD8 T cells from pregnant women show altered phenotype and function following H1N1/09 infection. J. Infect. Dis. 208(7): 1062-1070
Product options: 10

Product information

Size
Order no.
Price

CD1c (BDCA-1)-VioBright FITC, human

  • REA694
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-598
-

CD1c (BDCA-1)-VioBright FITC, human

  • REA694
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-540
-

CD1c (BDCA-1)-PE, human

  • REA694
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-594
-

CD1c (BDCA-1)-PE, human

  • REA694
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-536
-

CD1c (BDCA-1)-APC, human

  • REA694
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-595
-

CD1c (BDCA-1)-APC, human

  • REA694
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-537
-

CD1c (BDCA-1)-PE-Vio770, human

  • REA694
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-596
-

CD1c (BDCA-1)-PE-Vio770, human

  • REA694
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-538
-

CD1c (BDCA-1)-Biotin, human

  • REA694
  • 1:50
  • For research use only
for 30
tests
(1)
130-110-593
-

CD1c (BDCA-1)-Biotin, human

  • REA694
  • 1:50
  • For research use only
for 100
tests
(1)
130-110-535
-