CD197 (CCR7) antibodies, human

CD197 (CCR7) antibodies, human

Clone: REA108 | Dilution: 1:11
REA108 recognizes human CD197 which is a chemokine receptor with a C-C motif that mediates homing of T cells to secondary lymphoid organs via high endothelial venules (HEV) and is also known as CCR7. For example, naive T cells migrate very efficiently through lymph nodes using the adhesion molecules L-selectin, CD62L, and CCR7. Ligands for CCR7, e.g. CCL19, are expressed by the HEV of secondary lymphoid organs, by parenchymal cells within T cell zones of lymph nodes, and by endothelial cells at the openings of lymphatic vessels within peripheral tissues. Expression of CCR7 and the CD45RA isoform distinguishes three subsets of T cells: naive T cells (CCR7
+
CD45RA
+
), central memory T cells (CCR7
+
CD45RA
), and effector memory T cells (CCR7
CD45RA
).
Additional information: Clone REA108 displays negligible binding to Fc receptors.

Alternative Names

CCR7, BLR-2, CC-CKR-7, CCR-7, Cdw197, CMKBR7, EBI-1

Technical specifications

  • Antigen: CD197 (CCR7)
  • Clone: REA108
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: CCR7, BLR-2, CC-CKR-7, CCR-7, Cdw197, CMKBR7, EBI-1
  • Entrez Gene ID: 1236
  • Molecular mass of antigen [kDa]: 40
  • Distribution of antigen: B cells, dendritic cells, macrophages, NK cells, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, Biotin
  • Selected References

    1. Bacher, P. et al. (2014)
      Identification of immunogenic antigens from
      Aspergillus fumigatus
      by direct multi parameter characterization of specific conventional and regulatory CD4
      +
      T cells.
      J. Immunol. 193: 3332-3343
    2. Sallusto, F. et al. (1999) Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature 401: 708-712
    3. Baldan, W. et al. (2015) Efficient and reproducible generation of tumour-infiltrating lymphocytes for renal cell carcinoma. Br. J. Cancer 112(9): 1510-1518
    4. Alvarez-Fernández, C. et al. (2016) A short CD3/CD28 costimulation combined with IL-21 enhance the generation of human memory stem T cells for adoptive immunotherapy. J. Transl. Med. 14: 214
    5. Chang, Z. L. et al. (2015) Identification and selective expansion of functionally superior T cells expressing chimeric antigen receptors. J. Transl. Med. 13: 161
    6. Frascaroli, G. et al. (2018) Human Macrophages Escape Inhibition of Major Histocompatibility Complex-Dependent Antigen Presentation by Cytomegalovirus and Drive Proliferation and Activation of Memory CD4+ and CD8+ T Cells. Front Immunol 9
    7. Tilly, G. et al. (2017) IL-15 Harnesses Pro-inflammatory Function of TEMRA CD8 in Kidney-Transplant Recipients. Front Immunol 8

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA108
3D12++
TG8++
150503++
G043H7+
REA546+
FR11-11E8++
Product options: 6

Product information

Size
Order no.
Price

CD197 (CCR7)-PE, human

  • REA108
  • 1:11
  • For research use only
for 30
tests
(1)
130-099-362
-

CD197 (CCR7)-PE, human

  • REA108
  • 1:11
  • For research use only
for 100
tests
(1)
130-099-361
-

CD197 (CCR7)-APC, human

  • REA108
  • 1:11
  • For research use only
for 30
tests
(1)
130-099-364
-

CD197 (CCR7)-APC, human

  • REA108
  • 1:11
  • For research use only
for 100
tests
(1)
130-099-363
-

CD197 (CCR7)-Biotin, human

  • REA108
  • 1:11
  • For research use only
for 30
tests
(1)
130-099-154
-

CD197 (CCR7)-Biotin, human

  • REA108
  • 1:11
  • For research use only
for 100
tests
(1)
130-099-153
-

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