CD196 (CCR6) antibodies, human

CD196 (CCR6) antibodies, human

Clone: REA190 | Dilution: 1:11
Clone REA190 recognizes CD196 (CCR6), a seven transmembrane G protein–coupled receptor, which belongs to the family of beta chemokine receptors. Expression of CD196 is found on T cells, naive and memory B cells, and immature dendritic cells. CD196 selectivly binds chemokine MIP-3α/CCL20, which is secreted by a number of cells and tissues. In addition, members of a family of small cationic antimicrobial peptides, such as human β-defensins (hBD)-1 and -2, which are released by epithelial cells in response to microbial invasion or proinflammatory stimuli, are also shown to bind to CCR6. This interaction induce chemotaxis in immature dendritic cells and memory T cells.
Additional information: Clone REA190 displays negligible binding to Fc receptors.

Alternative Names

CCR6, BN-1, CKRL3, CMKBR6, DCR2, DRY6, GPR29, GPRCY4, STRL22

Technical specifications

  • Antigen: CD196 (CCR6)
  • Clone: REA190
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: CCR6, BN-1, CKRL3, CMKBR6, DCR2, DRY6, GPR29, GPRCY4, STRL22
  • Entrez Gene ID: 1235
  • Molecular mass of antigen [kDa]: 42
  • Distribution of antigen: B cells, T cells, dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, PE-Vio615, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA190 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA190
11A9++
R6H1++
TG7/CCR6++
G034E3+
  • Selected References

    1. Yang, D. et al. (1999) Beta-defensins: Linking innate and adaptive immunity through dendritic and T cell CCR6. Science 286(5439): 525-528
    2. Armas-González, E. et al. (2018) Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis. Arthritis Res. Ther. 20(1)
    3. Bergmann-Leitner, E. et al. (2018) Identification of Immune Signatures of Novel Adjuvant Formulations Using Machine Learning. Sci. Rep. (11): 17508
    4. Dorraji, E. et al. (2018) Mesenchymal stem cells and T cells in the formation of Tertiary Lymphoid Structures in Lupus Nephritis. Sci. Rep. 8(1)
    5. Farooq, F. et al. (2016) Circulating follicular T helper cells and cytokine profile in humans following vaccination with the rVSV-ZEBOV Ebola vaccine. Sci. Rep. 6: 27944
    6. Schutyser, E. et al. (2003) The CC chemokine CCL20 and its receptor CCR6. Cytokine Growth Factor Rev. 14(5): 409-426
Product options: 4
130-107-197

CD196 (CCR6)-PE-Vio615, human

for 30
tests
(1)
-
130-107-142

CD196 (CCR6)-PE-Vio615, human

for 100
tests
(1)
-
130-105-095

CD196 (CCR6)-Biotin, human

for 30
tests
(1)
-
130-105-065

CD196 (CCR6)-Biotin, human

for 100
tests
(1)
-

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