CD192 (CCR2) antibodies, human

CD192 (CCR2) antibodies, human

Clone: REA264 | Dilution: 1:50
Clone REA264 recognizes the CD192 antigen, a G-protein–linked seven transmembrane receptor which is also known as C-C chemokine receptor type 2 (CCR2) or monocyte chemoattractant protein 1 receptor (MCP-1-R). Two spliced variants of CD192 (CD192A and CD192B) are expressed on peripheral monocytes and basophils as a result of alternate splicing of a single gene and differ at the C-terminal end. CD192 and its main ligand CCL2 have been implicated in a wide range of immunobiological processes and neuropathologies, including recruitment of monocytes and regulation of bone marrow homeostasis, as well as multiple sclerosis, HIV-associated dementia, Alzheimer’s disease, and neuropathic pain.
Additional information: Clone REA264 displays negligible binding to Fc receptors.

Alternative names

CCR2, CC-CKR-2, CCR-2A, CCR2B, Ckr2, CKR2A, Ckr2b, Cmkbr2, MCP-1-R

Technical specifications

  • Antigen: CD192 (CCR2)
  • Clone: REA264
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: CCR2, CC-CKR-2, CCR-2A, CCR2B, Ckr2, CKR2A, Ckr2b, Cmkbr2, MCP-1-R
  • Entrez Gene ID: 1231
  • Molecular mass of antigen [kDa]: 42
  • Distribution of antigen: basophils, monocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE

Specificity

In order to compare the epitope specificity of REAfinity Clone REA264 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA264
REA624+
K03602+
48607++
  • Selected references

    1. Charo, I. F. et al. (1994) Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternative splicing of the carboxyl-terminal tails. Proc. Natl. Acad. Sci. U.S.A. 91(7): 2752-2756
    2. Henrich, T. J. et al. (2013) HIV-1 entry inhibitors: recent development and clinical use. Curr. Opin. Virol. 3(1): 51-57
Product options: 2
130-118-477

CD192 (CCR2)-PE, human

for 30
tests
(1)
-
130-118-338

CD192 (CCR2)-PE, human

for 100
tests
(1)
-