CD19 antibodies, mouse

CD19 antibodies, mouse

Clone: REA749 | Dilution: 1:50
Clone REA749 recognizes the mouse CD19 antigen, a type I transmembrane glycoprotein expressed on B cells throughout their development from the early pro–B cell through the mature B cell stages. Its expression is down-regulated during terminal differentiation to plasma cells. CD19 associates with complement receptor CD21 and with CD81 on the cell surface of mature B cells, thereby forming a multi-molecular complex which signals synergistically to membrane IgM. It is also expressed on follicular dendritic cells and peritoneal mast cells.
Additional information: Clone REA749 displays negligible binding to Fc receptors.

Alternative names

B4

Technical specifications

  • Antigen: CD19
  • Clone: REA749
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: B4
  • Entrez Gene ID: 12478
  • Molecular mass of antigen [kDa]: 58
  • Distribution of antigen: B cells, dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, VioBlue, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, VioBright 515, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA749 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA749
6D5++
1D3++
MB19-1++
  • Selected references

    1. Fearon, D. T. (1993) The CD19-CR2-TAPA-1 complex, CD45 and signaling by the antigen receptor of B lymphocytes. Curr. Opin. Immunol. 5(3): 341-348
    2. Tedder, T. F. et al. (1994) The CD19/CD21 signal transduction complex of B lymphocytes. Immunol. Today 15(9): 437-442
    3. Krop, I. et al. (1996) Self-renewal of B-1 lymphocytes is dependent on CD19. Eur. J. Immunol. 26(1): 238-242
  • Customer reports and application notes

Product options: 18
130-112-035

CD19-PE, mouse

30 µg in 200 µL (1)
-
130-111-883

CD19-PE, mouse

150 µg in 1 mL (1)
-
130-112-036

CD19-APC, mouse

30 µg in 200 µL (1)
-
130-111-884

CD19-APC, mouse

150 µg in 1 mL (1)
-
130-112-041

CD19-VioBlue, mouse

30 µg in 200 µL (1)
-
130-111-889

CD19-VioBlue, mouse

150 µg in 1 mL (1)
-
130-112-042

CD19-PE-Vio615, mouse

30 µg in 200 µL (1)
-
130-111-890

CD19-PE-Vio615, mouse

150 µg in 1 mL (1)
-
130-112-037

CD19-PE-Vio770, mouse

30 µg in 200 µL (1)
-
130-111-885

CD19-PE-Vio770, mouse

150 µg in 1 mL (1)
-
130-112-038

CD19-APC-Vio770, mouse

30 µg in 200 µL (1)
-
130-111-886

CD19-APC-Vio770, mouse

150 µg in 1 mL (1)
-
130-112-039

CD19-PerCP-Vio700, mouse

30 µg in 200 µL (1)
-
130-111-887

CD19-PerCP-Vio700, mouse

150 µg in 1 mL (1)
-
130-112-040

CD19-VioBright 515, mouse

30 µg in 200 µL (1)
-
130-111-888

CD19-VioBright 515, mouse

150 µg in 1 mL (1)
-
130-112-034

CD19-Biotin, mouse

30 µg in 200 µL (1)
-
130-111-882

CD19-Biotin, mouse

150 µg in 1 mL (1)
-