CD19 antibodies, human

CD19 antibodies, human

Clone: REA675 | Dilution: 1:11
REA675 recognizes the human CD19 antigen, a type I transmembrane glycoprotein of 95 kDa that belongs to the immunglobulin superfamily. CD19 is expressed on B cells throughout most stages of B cell differentiation, though its expression is down-regulated during their terminal differentiation to plasma cells. Expression of CD19 is also found in the majority of B cell–derived malignancies. CD19 is further present on follicular dendritic cells. On B cells, CD19 associates with CD21, CD81, and CD225 (Leu-13) forming a signal transduction complex.
Additional information: Clone REA675 displays negligible binding to Fc receptors.

Alternative Names

B4, Leu-12

Technical specifications

  • Antigen: CD19
  • Clone: REA675
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: B4, Leu-12
  • Distribution of antigen: B cells, dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, APC, VioGreen

Specificity

In order to compare the epitope specificity of REAfinity Clone REA675 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA675
SJ25C1++
HIB19++
LT19++
J3-119++
Product options: 5
130-110-349

CD19-FITC, human

for 30
tests
(1)
-
130-110-248

CD19-FITC, human

for 100
tests
(1)
-
130-110-351

CD19-APC, human

for 30
tests
(1)
-
130-110-355

CD19-VioGreen, human

for 30
tests
(1)
-
130-110-254

CD19-VioGreen, human

for 100
tests
(1)
-