CD184 (CXCR4) antibodies, human

CD184 (CXCR4) antibodies, human

Clone: REA649 | Dilution: 1:11
Clone REA649 recognizes the human CD184 antigen, a multi-pass membrane protein, also known as C-X-C chemokine receptor type 4 (CXCR4), leukocyte-derived seven transmembrane domain receptor (LESTR), or fusin. CD184 is ubiquitously expressed on blood and tissue cells. It mediates chemotaxis in mature and progenitor blood cells and is important for B lymphopoiesis, myelopoiesis, and cardiogenesis. CD184 exclusively interacts with the endogenous ligand CXCL12. Binding of CXCL12 transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Although CXCL12 is the only known chemokine that binds CD184, recent studies suggest that extracellular ubiquitin also acts as an immune modulator through CD184-mediated signaling. CD184 is a co-receptor for HIV entry by promoting Env-mediated fusion of the virus.
Additional information: Clone REA649 displays negligible binding to Fc receptors.

Alternative Names

CXCR4, Fusin, LESTR, FB22, HM89, LCR1, LAP-3, NPYRL

Technical specifications

  • Antigen: CD184 (CXCR4)
  • Clone: REA649
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: CXCR4, Fusin, LESTR, FB22, HM89, LCR1, LAP-3, NPYRL
  • Entrez Gene ID: 7852
  • Molecular mass of antigen [kDa]: 40
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
    ,
    rhesus monkey (
    Macaca mulatta
    )
    ,
    african green monkey (
    Chlorocebus aethiops
    )
    , baboon,
    chimpanzee (
    Pan troglodytes
    )
    ,
    sooty mangabey (
    Cercocebus atys
    )
  • Distribution of antigen: B cells, brain, dendritic cells, endothelial cells, epithelial cells, granulocytes, heart, hematopoietic stem cells, kidney, leukocytes, liver, lung, lymphocytes, megakaryocytes, mesenchymal stem cells, monocytes, myeloid cells, neurons, pancreatic carcinoma cells, placenta, platelets, skeletal muscle, spleen, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, APC, PE-Vio615, PE-Vio770
REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 
Other clonesOverlap in epitope recognition with REA649
12G5++
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