CD184 (CXCR4) antibodies, human

CD184 (CXCR4) antibodies, human

Clone: REA649 | Dilution: 1:11
Clone REA649 recognizes the human CD184 antigen, a multi-pass membrane protein, also known as C-X-C chemokine receptor type 4 (CXCR4), leukocyte-derived seven transmembrane domain receptor (LESTR), or fusin. CD184 is ubiquitously expressed on blood and tissue cells. It mediates chemotaxis in mature and progenitor blood cells and is important for B lymphopoiesis, myelopoiesis, and cardiogenesis. CD184 exclusively interacts with the endogenous ligand CXCL12. Binding of CXCL12 transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Although CXCL12 is the only known chemokine that binds CD184, recent studies suggest that extracellular ubiquitin also acts as an immune modulator through CD184-mediated signaling. CD184 is a co-receptor for HIV entry by promoting Env-mediated fusion of the virus.
Additional information: Clone REA649 displays negligible binding to Fc receptors.

Alternative Names

CXCR4, Fusin, LESTR, FB22, HM89, LCR1, LAP-3, NPYRL

Technical specifications

  • Antigen: CD184 (CXCR4)
  • Clone: REA649
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: CXCR4, Fusin, LESTR, FB22, HM89, LCR1, LAP-3, NPYRL
  • Entrez Gene ID: 7852
  • Molecular mass of antigen [kDa]: 40
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
    ,
    rhesus monkey (
    Macaca mulatta
    )
    ,
    african green monkey (
    Chlorocebus aethiops
    )
    , baboon,
    chimpanzee (
    Pan troglodytes
    )
    ,
    sooty mangabey (
    Cercocebus atys
    )
  • Distribution of antigen: B cells, brain, dendritic cells, endothelial cells, epithelial cells, granulocytes, heart, hematopoietic stem cells, kidney, leukocytes, liver, lung, lymphocytes, megakaryocytes, mesenchymal stem cells, monocytes, myeloid cells, neurons, pancreatic carcinoma cells, placenta, platelets, skeletal muscle, spleen, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, APC, PE-Vio615, PE-Vio770

Specificity

In order to compare the epitope specificity of REAfinity Clone REA649 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA649
12G5++
  • Selected References

    1. Loetscher, M. et al. (1994) Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes. J. Biol. Chem. 269(1): 232-237
    2. McKnight, A. et al. (1997) Inhibition of human immunodeficiency virus fusion by a monoclonal antibody to a coreceptor (CXCR4) is both cell type and virus strain dependent. J. Virol. 71(2): 1692-1696
    3. McKnight, A. et al. (1997) Inhibition of human immunodeficiency virus fusion by a monoclonal antibody to a coreceptor (CXCR4) is both cell type and virus strain dependent. J. Virol. 71(2): 1692-1696
    4. Berger, E. A. et al. (1999) Chemokine receptors as HIV-1 coreceptors: roles in viral entry, tropism, and disease. Annu. Rev. Immunol. 17: 657-700
    5. Loetscher, P. et al. (2000) Chemokines and their receptors in lymphocyte traffic and HIV infection. Adv. Immunol. 74: 127-180
    6. Ichiyama, K. et al. (2003) A duodenally absorbable CXC chemokine receptor 4 antagonist, KRH-1636, exhibits a potent and selective anti-HIV-1 activity. Proc. Natl. Acad. Sci. U.S.A. 100: 4185-4190
    7. Chatterjee, S. et al. (2014) The intricate role of CXCR4 in cancer. Adv. Cancer Res. 124: 31-82
    8. Chen, Y. et al. (2015) CXCR4 inhibition in tumor microenvironment facilitates anti-programmed death receptor-1 immunotherapy in sorafenib-treated hepatocellular carcinoma in mice. Hepatology 61(5): 1591-1602
Product options: 6
130-109-886

CD184 (CXCR4)-APC, human

for 30
tests
(1)
-
130-109-844

CD184 (CXCR4)-APC, human

for 100
tests
(1)
-
130-109-890

CD184 (CXCR4)-PE-Vio615, human

for 30
tests
(1)
-
130-109-848

CD184 (CXCR4)-PE-Vio615, human

for 100
tests
(1)
-
130-109-887

CD184 (CXCR4)-PE-Vio770, human

for 30
tests
(1)
-
130-109-845

CD184 (CXCR4)-PE-Vio770, human

for 100
tests
(1)
-

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