CD183 (CXCR3) antibodies, human

CD183 (CXCR3) antibodies, human

Clone: REA232 | Dilution: 1:11
Clone REA232 recognizes CD183, a 38 kDa G protein–coupled chemokine receptor. CD183 harbors seven-transmembrane α-helices, an extracellular N-terminal domain containing three loops involved in the chemokine ligand binding, an intracellular C-terminal domain involved in signal transduction. CD183 binds CXCL9, CXCL10, and CXCL11 as ligands. CD183-ligand interactions play role in diverse cellular functions such as chemotactic migration, cell proliferation, and death. Expression of CD183 is found on T cells, human airway epithelial cells, NK cells, plasmacytoid dendritic cells, mast cells, alveolar macrophages, and eosinophils.
Additional information: Clone REA232 displays negligible binding to Fc receptors.

Alternative Names

CXCR3, CD183, CKR-L2, CMKAR3, GPR9, IP10-R, Mig-R, MigR

Technical specifications

  • Antigen: CD183 (CXCR3)
  • Clone: REA232
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: CXCR3, CD183, CKR-L2, CMKAR3, GPR9, IP10-R, Mig-R, MigR
  • Distribution of antigen: T cells, epithelial cells, NK cells, mast cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, PE-Vio770, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA232 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA232
1C6/CXCR3++
CEW33D++
G025H7++
  • Selected References

    1. Hu, J. K. et al. (2011) Expression of chemokine receptor CXCR3 on T cells affects the balance between effector and memory CD8 T-cell generation. Proc. Natl. Acad. Sci. U.S.A. 108: E118-E127
    2. Colvin, R. A. et al. (2004) Intracellular domains of CXCR3 that mediate CXCL9, CXCL10, and CXCL11 function. J. Biol. Chem. 279: 30219-30227
    3. Bergmann-Leitner, E. et al. (2018) Identification of Immune Signatures of Novel Adjuvant Formulations Using Machine Learning. Sci. Rep. (11): 17508
    4. Farooq, F. et al. (2016) Circulating follicular T helper cells and cytokine profile in humans following vaccination with the rVSV-ZEBOV Ebola vaccine. Sci. Rep. 6: 27944
Product options: 8
130-101-380

CD183 (CXCR3)-PE, human

for 30
tests
(1)
-
130-101-379

CD183 (CXCR3)-PE, human

for 100
tests
(1)
-
130-101-378

CD183 (CXCR3)-APC, human

for 30
tests
(1)
-
130-101-377

CD183 (CXCR3)-APC, human

for 100
tests
(1)
-
130-101-382

CD183 (CXCR3)-PE-Vio770, human

for 30
tests
(1)
-
130-101-381

CD183 (CXCR3)-PE-Vio770, human

for 100
tests
(1)
-
130-106-179

CD183 (CXCR3)-Biotin, human

for 30
tests
(1)
-
130-106-128

CD183 (CXCR3)-Biotin, human

for 100
tests
(1)
-

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