CD169 (Siglec-1) antibodies, mouse

CD169 (Siglec-1) antibodies, mouse

Clone: REA197 | Dilution: 1:10
Clone REA197 recognizes the mouse CD169 antigen, a Single-pass type I membrane protein also known as Sialoadhesin or Sialic acid-binding Ig-like lectin 1 (Siglec-1). CD169 is a macrophage-restricted cell surface receptor that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8
+
T-cells. It is a member of the sialic acid-binding IgG-like lectin (Siglec) family of proteins characterized by affinity to specifically sialylated ligands, and under normal conditions is expressed on subsets of macrophages in secondary lymphoid tissues, such as lymph node and spleen. However, CD169
+
macrophages can also be found in a variety of pathological conditions, including (autoimmune) inflammatory infiltrates and tumors. CD169 has been shown to contribute to sialylated pathogen uptake, antigen presentation and lymphocyte proliferation, and to influence both immunity and tolerance. Additionally, CD169 positive macrophages, along with mesenchymal stem cells and β-adrenergic neurons, form the hematopoietic stem cell niche in the bone marrow. CD169
+
macrophages mediate signaling between the various cells and seem to promote hematopoietic stem cell retention to the niche.
Additional information: Clone REA197 displays negligible binding to Fc receptors.

Alternative Names

Siglec-1, SN, Sialic acid-binding Ig-like lectin 1 (Siglec-1)

Technical specifications

  • Antigen: CD169 (Siglec-1)
  • Clone: REA197
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: Siglec-1, SN, Sialic acid-binding Ig-like lectin 1 (Siglec-1)
  • Entrez Gene ID: 20612
  • Molecular mass of antigen [kDa]: 181
  • Distribution of antigen: macrophages, spleen
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA197 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA197
3D6.112++
Ser-4-
645608-
  • Selected References

    1. Saunderson, S. C. et al. (2014) CD169 mediates the capture of exosomes in spleen and lymph node Blood 123(2): 208-216
    2. Chow, A. et al. (2011) Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche J. Exp. Med. 208(2): 261-271
    3. Crocker, P. R. et al. (1994) Sialoadhesin, a macrophage sialic acid binding receptor for haemopoietic cells with 17 immunoglobulin-like domains EMBO J. 13(19): 4490-4503
Product options: 6
130-105-005

CD169 (Siglec-1)-PE, mouse

9 µg in 300 µL (1)
-
130-104-953

CD169 (Siglec-1)-PE, mouse

30 µg in 1 mL (1)
-
130-105-006

CD169 (Siglec-1)-APC, mouse

9 µg in 300 µL (1)
-
130-104-954

CD169 (Siglec-1)-APC, mouse

30 µg in 1 mL (1)
-
130-105-004

CD169 (Siglec-1)-Biotin, mouse

9 µg in 300 µL (1)
-
130-104-952

CD169 (Siglec-1)-Biotin, mouse

30 µg in 1 mL (1)
-