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Human peripheral blood mononuclear cells (PBMCs) were stained with CD164 antibodies or with the corresponding isotype control antibodies (left peak) and analyzed by flow cytometry using the MACSQuant®
cells were pre-gated for the analysis. The FcR Blocking Reagent has been used to avoid Fc receptor-mediated antibody labeling. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.