CD163 antibodies, human

CD163 antibodies, human

Clone: REA812 | Dilution: 1:50
Clone REA812 recognizes the human CD163 antigen, a single-chain transmembrane protein also known as hemoglobin scavenger receptor or M130. It is expressed by mature tissue macrophages and peripheral blood monocytes. The expression of CD163 is up-regulated
in vitro
and
in vivo
by anti-inflammatory mediators such as interleukin 10 (IL-10) and (gluco)corticosteroid and is shed to blood upon inflammatory activation of macrophages. CD163 functions as a high affinity scavenger receptor for the complex of haemoglobin and haptoglobin. Depending on the ligand, crosslinking of CD163 initiates signal transduction leading to the production of proinflammatory cytokines Il-1ß, IL-6, and GM-CSF or the anti-inflammatory cytokine IL-10.
Additional information: Clone REA812 displays negligible binding to Fc receptors.

Alternative names

GHI/61, M130, MM130

Technical specifications

  • Antigen: CD163
  • Clone: REA812
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: GHI/61, M130, MM130
  • Entrez Gene ID: 9332
  • Molecular mass of antigen [kDa]: 121
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Distribution of antigen: bone marrow, CNS cells, liver, macrophages, monocytes, spleen, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin
  • Selected references

    1. Pulford, K. et al. (1992) A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4. Immunology 75(4): 588-595
    2. Law, S. K. et al. (1993) A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. Eur. J. Immunol. 23(9): 2320-2325
    3. Ritter, M. et al. (2001) Interaction of CD163 with the regulatory subunit of casein kinase II (CKII) and dependence of CD163 signaling on CKII and protein kinase C. Eur. J. Immunol. 31(4): 999-1009
    4. Kristiansen, M. et al. (2001) Identification of the haemoglobin scavenger receptor. Nature 409(6817): 198-201
    5. Burdo, T. H. et al. (2011) Soluble CD163 made by monocyte/macrophages is a novel marker of HIV activity in early and chronic infection prior to and after anti-retroviral therapy. J. Infect. Dis. 204: 154-163
Product options: 17
130-112-290

CD163-FITC, human

for 30
tests
(1)
-
130-112-132

CD163-FITC, human

for 100
tests
(1)
-
130-112-286

CD163-PE, human

for 30
tests
(1)
-
130-112-128

CD163-PE, human

for 100
tests
(1)
-
130-112-287

CD163-APC, human

for 30
tests
(1)
-
130-112-129

CD163-APC, human

for 100
tests
(1)
-
130-112-292

CD163-VioBlue, human

for 30
tests
(1)
-
130-112-134

CD163-VioBlue, human

for 100
tests
(1)
-
130-112-284

CD163-PE-Vio615, human

for 30
tests
(1)
-
130-112-126

CD163-PE-Vio615, human

for 100
tests
(1)
-
130-112-288

CD163-PE-Vio770, human

for 30
tests
(1)
-
130-112-130

CD163-PE-Vio770, human

for 100
tests
(1)
-
130-112-131

CD163-APC-Vio770, human

for 100
tests
(1)
-
130-112-291

CD163-PerCP-Vio700, human

for 30
tests
(1)
-
130-112-133

CD163-PerCP-Vio700, human

for 100
tests
(1)
-
130-112-283

CD163-Biotin, human

for 30
tests
(1)
-
130-112-125

CD163-Biotin, human

for 100
tests
(1)
-