CD159a (NKG2A) antibodies, human

CD159a (NKG2A) antibodies, human

Clone: REA110 | Dilution: 1:50
Clone REA110 recognizes CD159a, an inhibitory natural killer (NK) cell receptor. CD159a forms heterodimer with CD94 and contains a C-type lectin ectodomain. Inhibitory signal is transmitted by the heterodimer via immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and upon ligand engagement, ITIMs are phosphorylated and transmit signal through docking of various tyrosine phosphatases. CD159a/CD94 binds non-classical MHC class I protein, HLA-E. Expression of CD159a is found mainly on NK cells and on subsets of CD8
+
cells.
Additional information: Clone REA110 displays negligible binding to Fc receptors.

Alternative Names

KLRC1, CD159a, NKG2, NKG2A

Technical specifications

  • Antigen: CD159a (NKG2A)
  • Clone: REA110
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: KLRC1, CD159a, NKG2, NKG2A
  • Entrez Gene ID: 3821
  • Molecular mass of antigen [kDa]: 26
  • Cross-reactivity:
    chimpanzee (
    Pan troglodytes
    )
    ,
    olive baboon (
    Papio anubis
    )
    ,
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Distribution of antigen: NK cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, VioBright FITC, PE, APC, PE-Vio770, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA110 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA110
131411+
Z199++
  • Selected References

    1. Rueda, C. et al. (2016)
      Lipopolysaccharide-induced chorioamnionitis promotes IL-1-dependent inflammatory FOXP3
      +
      CD4
      +
      T cells in the fetal rhesus macaque.
      J. Immunol. 196(9): 3706-3715
    2. Mahaweni, N. et al. (2018)
      NKG2A expression is not
      per se
      detrimental for the anti-multiple myeloma activity of activated natural killer cells in an
      in vitro
      system mimicking the tumor microenvironment.
      Front Immunol 9: 1415
    3. Jacomet, F. et al. (2017)
      The hypothesis of the human iNKT/innate CD8
      +
      T-cell axis applied to cancer: evidence for a deficiency in chronic myeloid leukemia.
      Front Immunol 7: 688
    4. Croci, S. et al. (2018) Higher frequencies of lymphocytes expressing the natural killer group 2D receptor in patients with Behçet disease. Front Immunol 9: 2157
    5. Borrego, F. et al. (2006) The CD94/NKG2 family of receptors: from molecules and cells to clinical relevance. Immunol. Res. 35(3): 263-278
    6. Wolpert, F. et al. (2015) Interferon-β modulates the innate immune response against glioblastoma initiating cells. PLoS One 10(10): e0139603
    7. Veluchamy, J. P. et al. (2017) High-efficiency lysis of cervical cancer by allogeneic NK cells derived from umbilical cord progenitors is independent of HLA status. Cancer Immunol. Immunother. 66: 51-61
    8. Reeves R. K. et al. (2015) Antigen-specific NK cell memory in rhesus macaques. Nat. Immunol. 16: 927-932
    9. Sullivan, L. C. et al. (2007) The heterodimeric assembly of the CD94-NKG2 receptor family and implications for human leukocyte antigen-E recognition. Immunity 27(6): 900-911
    10. Boyington, J. C. et al. (1999) Structure of CD94 reveals a novel C-type lectin fold: implications for the NK cell-associated CD94/NKG2 receptors. Immunity 10(1): 75-82
    11. Rechtien, A. et al. (2017) Systems vaccinology identifies an early innate immune signature as a correlate of antibody responses to the Ebola vaccine rVSV-ZEBOV. Cell Rep 20(9): 2251-2261
Product options: 12
130-114-091

CD159a (NKG2A)-FITC, human

for 30
tests
(1)
-
130-113-565

CD159a (NKG2A)-FITC, human

for 100
tests
(1)
-
130-114-094

CD159a (NKG2A)-VioBright FITC, human

for 30
tests
(1)
-
130-113-568

CD159a (NKG2A)-VioBright FITC, human

for 100
tests
(1)
-
130-114-092

CD159a (NKG2A)-PE, human

for 30
tests
(1)
-
130-113-566

CD159a (NKG2A)-PE, human

for 100
tests
(1)
-
130-114-089

CD159a (NKG2A)-APC, human

for 30
tests
(1)
-
130-113-563

CD159a (NKG2A)-APC, human

for 100
tests
(1)
-
130-114-093

CD159a (NKG2A)-PE-Vio770, human

for 30
tests
(1)
-
130-113-567

CD159a (NKG2A)-PE-Vio770, human

for 100
tests
(1)
-
130-114-090

CD159a (NKG2A)-Biotin, human

for 30
tests
(1)
-
130-113-564

CD159a (NKG2A)-Biotin, human

for 100
tests
(1)
-